Calpain 13 antibody - Domain I, large subunit (ab28235)

Product Name 

Calpain 13 antibody - Domain I, large subunit

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 13 - Domain I, large subunit

Immunogen 

Synthetic peptide based on the aminoterminal end of domain III in the large subunit of Human Calpain 13.(Peptide available as ab41370.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab28235 does not cross react with the other calpain family members (calpain 1, Calpain 2, ncl 2, ncl 3, etc.).

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 13 antibodies

Epitope 

Aminoterminal end of domain-III in the large subunit of Calpain 13

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates - 1/5000 for chemiluminescent substrates. Predicted molecular weight: 77 kDa. But note that when used against the reduced protein, ab28235 identifies bands at 80 Kd, and a series of further cleaved forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Intracellular

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain

Relevance 

Calpain 13 is an intracellular, calcium-dependent cysteine protease. Calpain 13 was first described in human EST databases, and maps to mouse chromosome 2. The different isoforms differ in the carboxyterminal ends, ending with aberrant domain-III and lacking domain IV. Calpain 13 looks similar to the other calpain members in domains I and II, but domain-III lacks the polyglutamic acid stretch that aids in calcium binding, and domain-IV is very different. Sequence alignments place Calpain 13 closest to calpain 14, also found on chromosome 2.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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