Calpain 14 antibody - Domain I, large subunit (ab38945)

Product Name 

Calpain 14 antibody - Domain I, large subunit

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 14 - Domain I, large subunit

Immunogen 

Synthetic peptide based on the aminoterminal end of domain-I in the large subunit of Human Calpain 14.(Peptide available as ab41371.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab38945 recognizes latent but not the aminotruncated (active) Calpain 14. It binds to Calpain 14, but does not cross react with the other calpain family members (Calpain 1, Calpain 2, ncl 2, ncl 3, etc.)

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 14 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. When used against the reduced protein this antibody identifies bands at 76 kD, and a series of further cleaved forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Neuroscience >> Neurodegeneration >> Other

Relevance 

Calpains are used as markers of traumatic brain injury (Pineda, 2004). Calpain 14 is an intracellular, calcium-dependent cysteine protease. Calpain 14 was first described in human EST databases, and maps to mouse chromosome 2. The 663 amino acid sequence predicts a protein of 76.749 kD, with a isoelectric point of 8.587 (basic compared to the acidic isoelectric point of “classical” calpains). Calpain 14 looks similar to the other calpain members in domains I and II, but domain III lacks the polyglutamic acid stretch that aids in calcium binding, and domain IV is very different. Sequence alignments place Calpain-14 closest to calpain 13, also found on chromosome 2.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.5% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab38945 has been peptide-affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Store diluted antibody at 4°C with 0.05% Sodium Azide as preservative.

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