The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 115 kDa and a series of further cleaved forms. (predicted molecular weight: 117 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityWidely expressed with higher expression in brain.
Sequence similaritiesBelongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 5 RanBP2-type zinc fingers.