Calpain 15 antibody - Amino end, Zinc Finger Domain (ab38950)

Product Name 

Calpain 15 antibody - Amino end, Zinc Finger Domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 15 - Amino end, Zinc Finger Domain

Immunogen 

Synthetic peptide based on the aminoterminal end of domain-I in the large subunit of Human Calpain 15.(Peptide available as ab41373.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab38950 binds to Calpain 15, but does not cross react with the other calpain family members (calpain 1, Calpain 2, ncl 2, ncl 3, etc.) and recognizes latent but not the aminotruncated (active) Calpain 15.

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 15 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. When used against the reduced protein this antibody identifies bands at 115 kD (minor band), and a series of further cleaved forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Neuroscience >> Sensory Systems >> Vision
Signal Transduction >> Calcium Signaling >> Calpain

Relevance 

Calpain 15 is an intracellular, calcium dependent cysteine protease. Calpain 15 was first described as the human homologue of SOL, the small optic lobe protein, in drosophila. The Calpain 15 sequence is very different from the classical calpains, and has an extended aminoterminal domain. In fact, about the only similarity between this and the classical calpains is the catalytic residues, CHN, which are all conserved in Calpain 15. Sequence alignments place Calpain 15 closest to Calpain 7. The Calpain 15 sequence is 1086 amino acids long, predicting a protein of 117kD, much larger than other calpain family members (almost all of the difference is in the amino end).

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab38950 has been peptide-affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Store diluted antibody at 4°C with 0.05% Sodium Azide as preservative.

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