Anti-Calpain 2 antibody [1E8] (ab713)

Overview

  • Product nameAnti-Calpain 2 antibody [1E8]
    See all Calpain 2 primary antibodies
  • Description
    Mouse monoclonal [1E8] to Calpain 2
  • SpecificityRecognises a linear epitope of the 80 kDa (large) subunit of bovine m-calpain
  • Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Bovine cardiac Calpain 2.

Properties

Applications

Our Abpromise guarantee covers the use of ab713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 µg/ml. Fix with 2% paraformaldehyde.
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml.

Target

  • FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 3 EF-hand domains.
  • Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium activated neutral proteinase 2 antibody
    • Calcium activated neutral proteinase antibody
    • Calcium-activated neutral protease 2, catalytic subunit antibody
    • Calcium-activated neutral proteinase 2 antibody
    • CALP80 antibody
    • Calpain 2 (m/II) large subunit antibody
    • Calpain 2 catalytic subunit antibody
    • Calpain 2 large catalytic subunit antibody
    • Calpain 2 large subunit antibody
    • Calpain 2, large [catalytic] subunit antibody
    • Calpain large polypeptide L2 antibody
    • Calpain M type antibody
    • Calpain M-type antibody
    • Calpain-2 catalytic subunit antibody
    • Calpain-2 large subunit antibody
    • Calpain2 antibody
    • CAN2_HUMAN antibody
    • CANP 2 antibody
    • CANP L2 antibody
    • CANP2 antibody
    • CANPL 2 antibody
    • CANPL2 antibody
    • CANPml antibody
    • Capa2 antibody
    • CAPN 2 antibody
    • CAPN2 antibody
    • FLJ39928 antibody
    • M calpain antibody
    • M calpin antibody
    • M type antibody
    • M-calpain antibody
    • mCANP antibody
    • Millimolar calpain antibody
    • Millimolar-calpain antibody
    see all

Anti-Calpain 2 antibody [1E8] images

  • Immunodetection of m-calpain using MAb 1E8 (1) in Western blot.
  • Immunoflurescence of intracellular calpain in bovine lens epithel cells using MAb 1E8 (green).

References for Anti-Calpain 2 antibody [1E8] (ab713)

ab713 has not yet been referenced specifically in any publications.

Product Wall

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Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (chondrocytes and fibrochondrocytes)
Specification chondrocytes and fibrochondrocytes
Fixative Formaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2%
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Submitted Mar 09 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"