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Synthetic peptide based on the Carboxyterminal end of the large subunit of human calpain 2. (Peptide available as ab41303.)
Our Abpromise guarantee covers the use of ab28259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 80 kDa.Can be blocked with Calpain 2 peptide (ab41303). 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. But note that when used against the reduced protein, ab28259 identifies bands at 80 kDa, 58 kDa and a series of further cleaved active forms. Dilution optimised using Chromogenic detection.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"