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Synthetic peptide based on the amino terminal end of domain-III in the large subunit of Rat Calpain 2. (Peptide available as ab41302.)
Our Abpromise guarantee covers the use of ab39165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 76 kDa (predicted molecular weight: 80 kDa). Dilution optimised using Chromogenic detection.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17192410|
|IHC-P||Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"