Overview

  • Product name
  • Description
    Rabbit polyclonal to Calpain 2
  • Specificity
    ab39165 binds to reduced and non-reduced calpain 2, but does not cross react with the other calpain family members (m calpain, calpain 94, ncl 2, ncl 3, etc.). It reacts with latent and active (amino-truncated) Calpain 2.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide based on the amino terminal end of domain-III in the large subunit of Rat Calpain 2. (Peptide available as ab41302.)

  • Positive control
    • Cell lysate from human chondrosarcoma treated with IL1 alpha.

Properties

Applications

Our Abpromise guarantee covers the use of ab39165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 76 kDa (predicted molecular weight: 80 kDa). Dilution optimised using Chromogenic detection.
ICC/IF Use at an assay dependent concentration. PubMed: 17192410
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 3 EF-hand domains.
  • Cellular localization
    Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium activated neutral proteinase 2 antibody
    • Calcium activated neutral proteinase antibody
    • Calcium-activated neutral protease 2, catalytic subunit antibody
    • Calcium-activated neutral proteinase 2 antibody
    • CALP80 antibody
    • Calpain 2 (m/II) large subunit antibody
    • Calpain 2 catalytic subunit antibody
    • Calpain 2 large catalytic subunit antibody
    • Calpain 2 large subunit antibody
    • Calpain 2, large [catalytic] subunit antibody
    • Calpain large polypeptide L2 antibody
    • Calpain M type antibody
    • Calpain M-type antibody
    • Calpain-2 catalytic subunit antibody
    • Calpain-2 large subunit antibody
    • Calpain2 antibody
    • CAN2_HUMAN antibody
    • CANP 2 antibody
    • CANP L2 antibody
    • CANP2 antibody
    • CANPL 2 antibody
    • CANPL2 antibody
    • CANPml antibody
    • Capa2 antibody
    • CAPN 2 antibody
    • CAPN2 antibody
    • FLJ39928 antibody
    • M calpain antibody
    • M calpin antibody
    • M type antibody
    • M-calpain antibody
    • mCANP antibody
    • Millimolar calpain antibody
    • Millimolar-calpain antibody
    see all

Anti-Calpain 2 antibody images

  • All lanes : Anti-Calpain 2 antibody (ab39165) at 1/1000 dilution

    Lane 1 : Calpain 2 control
    Lane 2 : Cell lysates from human chondrosarcoma (treated with IL1 alpha) at 10 µg


    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 76 kDa (why is the actual band size different from the predicted?)
  • ab39165 (4µg/ml) staining CALPAIN2 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-Calpain 2 antibody (ab39165)

This product has been referenced in:
  • Zhu X  et al. The role of calpains in ventilator-induced diaphragm atrophy. Intensive Care Med Exp 5:14 (2017). Read more (PubMed: 28290154) »
  • Yin Y  et al. Accumulation of human full-length tau induces degradation of nicotinic acetylcholine receptor a4 via activating calpain-2. Sci Rep 6:27283 (2016). WB . Read more (PubMed: 27277673) »

See all 18 Publications for this product

Product Wall

Application
Western blot
Sample
Rat Tissue lysate - whole (brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
15 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Submitted Jun 19 2017

Application
Western blot
Sample
Rat Tissue lysate - whole (spleen)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
15 µg
Specification
spleen
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Submitted Apr 21 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (heart)
Permeabilization
Yes - 0.05% triton x-100 in PBS
Specification
heart
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C
Fixative
post fixed with 4% paraformaldehyde
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Submitted May 02 2016

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (T lymphocyte)
Specification
T lymphocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 17 2014

Thank you very much for your interest in ab39165.

To our knowledge ab39165 has not been tested in IP. Therefore, I can offer a discount off a future purchase if you buy ab39165 now, test it in IP and submit feedback to us in the form of an Ab...

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Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Vero cells)
Loading amount
120000 cells
Specification
Vero cells
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted May 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Loading amount
30 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Nov 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HUVEC)
Specification
HUVEC
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100
Blocking step
Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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Submitted Aug 06 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HUVEC)
Loading amount
10000 cells
Specification
HUVEC
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 06 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (RPE-J cell line, differentiated, seeded at 50%)
Loading amount
10 µg
Specification
RPE-J cell line, differentiated, seeded at 50%
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10%
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Submitted Mar 26 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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