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Rabbit polyclonal to Calpain 2
Predicted to work with:
Mouse, Sheep, Rabbit, Cow, Pig, Monkey
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Rat Calpain 2.
(Peptide available as
This antibody gave a positive signal in HeLa whole cell lysate as well as Rat Brain Tissue Lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain. Contains 3 EF-hand domains.
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
Information by UniProt
Calcium activated neutral proteinase 2 antibody
Calcium activated neutral proteinase antibody
Calcium-activated neutral protease 2, catalytic subunit antibody
Western blot - Calpain 2 antibody (ab88110)
All lanes : Anti-Calpain 2 antibody (ab88110) at 1 µg/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Brain (Rat) Tissue Lysate Lysates/proteins at 10 µg/ml per lane. Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 79 kDa Observed band size: 79 kDa Additional bands at: 150 kDa, 47 kDa, 85 kDa. We are unsure as to the identity of these extra bands. Exposure time: 4 minutes
Immunocytochemistry/ Immunofluorescence - Anti-Calpain 2 antibody (ab88110)
ICC/IF image of ab88110 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88110, 5µg/ml) overnight at +4°C. The secondary antibody (green) was
Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
has not yet been referenced specifically in any publications.
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