| | | | Immunocytochemistry/ Immunofluorescence-Calpain 2 antibody - Aminoterminal end domain I(ab39167)
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| Product Name | Calpain 2 antibody - Aminoterminal end domain I |
| | See all Calpain 2 antibodies (9)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to Calpain 2 - Aminoterminal end domain I |
| Immunogen | Synthetic peptide based on the amino terminal end of the large subunit (Domain I) of Human Calpain 2.
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| Reacts with
(species key) | Hu, Ms, Rat |
| Specificity | This antibody reacts with the propeptide domain, (Domain I) of Calpain 2, aminoterminal from the activation cleavage site. It binds to reduced and non reduced Calpain 2, but does not recognize amino processed Calpain 2. The antibody does not cross react with the other calpain family members (m calpain, calpain 94, ncl 2, ncl 3, etc.). We have a range of domain specific antibodies for this target. For a full list please see all Calpain 2 antibodies |
| Tested applications
(see key) | ICC/IF, WB |
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| Abreviews | |
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| Application notes
(see key) | Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Positive control
(see definition) | Cell lysates from human fetal fibroblasts. |
| Cellular localization | Cytoplasmic. Cell membrane. Translocates to the plasma membrane upon Ca2+ binding.
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| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain |
| Relevance | Mu Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names.
The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin.
Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
This antibody could be used to discriminate between latent and amino processed forms of Calpain 2, as it does not recognize amino processed Calpain 2. |
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| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calpain 2 antibody - Aminoterminal end domain I is on this datasheet. But please do contact us if you would like any reassurance! |
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| See below for Calpain 2 antibody - Aminoterminal end domain I images, references, products related to ab39167 and other tools. |
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Calpain 2 antibody - Aminoterminal end domain I images: |
 Immunocytochemistry/ Immunofluorescence-Calpain 2 antibody - Aminoterminal end domain I(ab39167)
ICC/IF image of ab39167 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39167, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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| Search PubMed (MEDLINE) for references to Calpain 2 |
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