Calpain 2 antibody - Aminoterminal end domain III (ab39165)

Product Name 

Calpain 2 antibody - Aminoterminal end domain III

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 2 - Aminoterminal end domain III

Immunogen 

Synthetic peptide based on the amino terminal end of domain-III in the large subunit of Rat Calpain 2.(Peptide available as ab41302.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab39165 binds to reduced and non-reduced calpain 2, but does not cross react with the other calpain family members (m calpain, calpain 94, ncl 2, ncl 3, etc.). It reacts with latent and active (amino-truncated) Calpain 2.

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 2 antibodies

Tested applications 
(see key)

ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 - 1/5000. Detects a band of approximately 76 kDa (predicted molecular weight: 80 kDa). Dilution optimised using Chromogenic detection.
ICC/IF: Use at an assay dependent dilution from (PubMed: 17192410).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Cell lysate from human chondrosarcoma treated with IL1 alpha.

Cellular localization 

Cytoplasmic. Cell membrane. Translocates to the plasma membrane upon Ca²⁺ binding.

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain

Relevance 

Mu Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names.
The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin.

Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

See below for Calpain 2 antibody - Aminoterminal end domain III images, references, products related to ab39165 and other tools.

Calpain 2 antibody - Aminoterminal end domain III images:

References for Calpain 2 antibody - Aminoterminal end domain III (ab39165)

  This product has been used in: (two most recent references)

De Maria A et al. Calpain expression and activity during lens fiber cell differentiation. J Biol Chem 284:13542-50 (2009). WB; Mouse. PubMed: 19269960

Ozaki T et al. Mitochondrial m-calpain plays a role in the release of truncated apoptosis-inducing factor from the mitochondria. Biochim Biophys Acta 1793:1848-59 (2009). WB; Rat. PubMed: 19833151

See all 3 publication references for this product.


If you publish research using ab39165 please let us know so that we can cite the reference on this datasheet.

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