Calpain 2 antibody - Domain IV (ab28259)

Product Name 

Calpain 2 antibody - Domain IV

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 2 - Domain IV

Immunogen 

Synthetic peptide based on the Carboxyterminal end of the large subunit of human calpain 2.(Peptide available as ab41303.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab28259 recognises the Carboxyterminal end of the large subunit of calpain 2, both in its latent and aminoprocessed (activated) forms. It does not recognise the other calpain family members (calpain 1, calpain 94, ncl 2, ncl 3, etc.).

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 2 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Predicted molecular weight: 80 kDa. But note that when used against the reduced protein, ab28259 identifies bands at 80 kDa, 58 kDa and a series of further cleaved active forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic. Cell membrane. Translocates to the plasma membrane upon Ca²⁺ binding.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain

Relevance 

Mu Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names.
The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin.

Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

See below for Calpain 2 antibody - Domain IV images, references, products related to ab28259 and other tools.

Calpain 2 antibody - Domain IV images:

Customer reviews (feedback) regarding Calpain 2 antibody - Domain IV

Customer FAQs regarding Calpain 2 antibody - Domain IV

Protocols for Calpain 2 antibody - Domain IV

Price and availability of products related to Calpain 2 antibody - Domain IV

Calpain 2 antibody - Domain IV - related products: