• Product name
    Anti-Calpain 2 antibody [EPR2562Y]
    See all Calpain 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2562Y] to Calpain 2
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues within human Calpain 2.

  • Positive control
    • HeLa cytoplasmic or membrane lysate Permeabilized HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents


Associated products


Our Abpromise guarantee covers the use of ab75994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 80 kDa.
IP 1/20.
Flow Cyt 1/60. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
    • Tissue specificity
    • Sequence similarities
      Belongs to the peptidase C2 family.
      Contains 1 calpain catalytic domain.
      Contains 3 EF-hand domains.
    • Cellular localization
      Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calcium activated neutral proteinase 2 antibody
      • Calcium activated neutral proteinase antibody
      • Calcium-activated neutral protease 2, catalytic subunit antibody
      • Calcium-activated neutral proteinase 2 antibody
      • CALP80 antibody
      • Calpain 2 (m/II) large subunit antibody
      • Calpain 2 catalytic subunit antibody
      • Calpain 2 large catalytic subunit antibody
      • Calpain 2 large subunit antibody
      • Calpain 2, large [catalytic] subunit antibody
      • Calpain large polypeptide L2 antibody
      • Calpain M type antibody
      • Calpain M-type antibody
      • Calpain-2 catalytic subunit antibody
      • Calpain-2 large subunit antibody
      • Calpain2 antibody
      • CAN2_HUMAN antibody
      • CANP 2 antibody
      • CANP L2 antibody
      • CANP2 antibody
      • CANPL 2 antibody
      • CANPL2 antibody
      • CANPml antibody
      • Capa2 antibody
      • CAPN 2 antibody
      • CAPN2 antibody
      • FLJ39928 antibody
      • M calpain antibody
      • M calpin antibody
      • M type antibody
      • M-calpain antibody
      • mCANP antibody
      • Millimolar calpain antibody
      • Millimolar-calpain antibody
      see all

    Anti-Calpain 2 antibody [EPR2562Y] images

    • Anti-Calpain 2 antibody [EPR2562Y] (ab75994) at 1/500 dilution + HeLa cell lysate at 10 µg

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized HeLa cells using ab75994 (red) or a rabbit IgG (negative) (green).

    References for Anti-Calpain 2 antibody [EPR2562Y] (ab75994)

    This product has been referenced in:
    • Sun L  et al. GAS2-Calpain2 axis contributes to the growth of leukemic cells. Acta Biochim Biophys Sin (Shanghai) 47:795-804 (2015). Read more (PubMed: 26358320) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab75994.
    Please use the links above to contact us or submit feedback about this product.


    Sign up