Overview

  • Product name
    Anti-Calpain 2 antibody [EPR2562Y]
    See all Calpain 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2562Y] to Calpain 2
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues within human Calpain 2.

  • Positive control
    • HeLa cytoplasmic or membrane lysate Permeabilized HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 80 kDa.
IP 1/20.
Flow Cyt 1/60. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the peptidase C2 family.
      Contains 1 calpain catalytic domain.
      Contains 3 EF-hand domains.
    • Cellular localization
      Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calcium activated neutral proteinase 2 antibody
      • Calcium activated neutral proteinase antibody
      • Calcium-activated neutral protease 2, catalytic subunit antibody
      • Calcium-activated neutral proteinase 2 antibody
      • CALP80 antibody
      • Calpain 2 (m/II) large subunit antibody
      • Calpain 2 catalytic subunit antibody
      • Calpain 2 large catalytic subunit antibody
      • Calpain 2 large subunit antibody
      • Calpain 2, large [catalytic] subunit antibody
      • Calpain large polypeptide L2 antibody
      • Calpain M type antibody
      • Calpain M-type antibody
      • Calpain-2 catalytic subunit antibody
      • Calpain-2 large subunit antibody
      • Calpain2 antibody
      • CAN2_HUMAN antibody
      • CANP 2 antibody
      • CANP L2 antibody
      • CANP2 antibody
      • CANPL 2 antibody
      • CANPL2 antibody
      • CANPml antibody
      • Capa2 antibody
      • CAPN 2 antibody
      • CAPN2 antibody
      • FLJ39928 antibody
      • M calpain antibody
      • M calpin antibody
      • M type antibody
      • M-calpain antibody
      • mCANP antibody
      • Millimolar calpain antibody
      • Millimolar-calpain antibody
      see all

    Images

    • Anti-Calpain 2 antibody [EPR2562Y] (ab75994) at 1/500 dilution + HeLa cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized HeLa cells using ab75994 (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:
    • Sun L  et al. GAS2-Calpain2 axis contributes to the growth of leukemic cells. Acta Biochim Biophys Sin (Shanghai) 47:795-804 (2015). Read more (PubMed: 26358320) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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