Anti-Calpain 2 antibody [EPR2562Y] (ab75994)


  • Product nameAnti-Calpain 2 antibody [EPR2562Y]
    See all Calpain 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2562Y] to Calpain 2
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues within human Calpain 2.

  • Positive control
    • HeLa cytoplasmic or membrane lysate Permeabilized HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Associated products


Our Abpromise guarantee covers the use of ab75994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 80 kDa.
IP 1/20.
Flow Cyt 1/60. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the peptidase C2 family.
      Contains 1 calpain catalytic domain.
      Contains 3 EF-hand domains.
    • Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calcium activated neutral proteinase 2 antibody
      • Calcium activated neutral proteinase antibody
      • Calcium-activated neutral protease 2, catalytic subunit antibody
      • Calcium-activated neutral proteinase 2 antibody
      • CALP80 antibody
      • Calpain 2 (m/II) large subunit antibody
      • Calpain 2 catalytic subunit antibody
      • Calpain 2 large catalytic subunit antibody
      • Calpain 2 large subunit antibody
      • Calpain 2, large [catalytic] subunit antibody
      • Calpain large polypeptide L2 antibody
      • Calpain M type antibody
      • Calpain M-type antibody
      • Calpain-2 catalytic subunit antibody
      • Calpain-2 large subunit antibody
      • Calpain2 antibody
      • CAN2_HUMAN antibody
      • CANP 2 antibody
      • CANP L2 antibody
      • CANP2 antibody
      • CANPL 2 antibody
      • CANPL2 antibody
      • CANPml antibody
      • Capa2 antibody
      • CAPN 2 antibody
      • CAPN2 antibody
      • FLJ39928 antibody
      • M calpain antibody
      • M calpin antibody
      • M type antibody
      • M-calpain antibody
      • mCANP antibody
      • Millimolar calpain antibody
      • Millimolar-calpain antibody
      see all

    Anti-Calpain 2 antibody [EPR2562Y] images

    • Anti-Calpain 2 antibody [EPR2562Y] (ab75994) at 1/500 dilution + HeLa cell lysate at 10 µg

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized HeLa cells using ab75994 (red) or a rabbit IgG (negative) (green).

    References for Anti-Calpain 2 antibody [EPR2562Y] (ab75994)

    ab75994 has not yet been referenced specifically in any publications.

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