Anti-Calpain 2 antibody [EPR2562Y] (ab75994)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Calpain 2 antibody [EPR2562Y]
See all Calpain 2 primary antibodies -
DescriptionRabbit monoclonal [EPR2562Y] to Calpain 2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P -
Species reactivityReacts with: Human
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Immunogen
A synthetic peptide corresponding to residues within human Calpain 2.
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Positive control
- HeLa cytoplasmic or membrane lysate Permeabilized HeLa cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR2562Y
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab75994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Detects a band of approximately 80 kDa. | |
| IP | 1/20. | |
| Flow Cyt | 1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
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Tissue specificityUbiquitous.
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Sequence similaritiesBelongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains. -
Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
- Information by UniProt
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Database links
- Entrez Gene: 824 Human
- Omim: 114230 Human
- SwissProt: P17655 Human
- Unigene: 350899 Human
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Alternative names
- Calcium activated neutral proteinase 2 antibody
- Calcium activated neutral proteinase antibody
- Calcium-activated neutral protease 2, catalytic subunit antibody
see all
Images
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![Western blot - Anti-Calpain 2 antibody [EPR2562Y] (ab75994)](http://a.abcam.com/ps/products/75/ab75994/Images/ab75994_WB_1.jpg)
Western blot - Anti-Calpain 2 antibody [EPR2562Y] (ab75994)Anti-Calpain 2 antibody [EPR2562Y] (ab75994) at 1/500 dilution + HeLa cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Observed band size: 75 kDa (why is the actual band size different from the predicted?) -
![Flow Cytometry - Anti-Calpain 2 antibody [EPR2562Y] (ab75994)](http://a.abcam.com/ps/products/75/ab75994/Images/ab75994_FC_1.jpg)
Flow Cytometry - Anti-Calpain 2 antibody [EPR2562Y] (ab75994)Flow cytometric analysis of permeabilized HeLa cells using ab75994 (red) or a rabbit IgG (negative) (green).
References
This product has been referenced in:
- Sun L et al. GAS2-Calpain2 axis contributes to the growth of leukemic cells. Acta Biochim Biophys Sin (Shanghai) 47:795-804 (2015). Read more (PubMed: 26358320) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"