Recombinant
RabMAb

Anti-Calpain 2 antibody [EPR5977] (ab126600)

Overview

  • Product name
    Anti-Calpain 2 antibody [EPR5977]
    See all Calpain 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR5977] to Calpain 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Calpain 2 aa 650-750. The exact sequence is proprietary.

  • Positive control
    • PC3, HeLa, A431 and 293T cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).

Target

  • Function
    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 3 EF-hand domains.
  • Cellular localization
    Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium activated neutral proteinase 2 antibody
    • Calcium activated neutral proteinase antibody
    • Calcium-activated neutral protease 2, catalytic subunit antibody
    • Calcium-activated neutral proteinase 2 antibody
    • CALP80 antibody
    • Calpain 2 (m/II) large subunit antibody
    • Calpain 2 catalytic subunit antibody
    • Calpain 2 large catalytic subunit antibody
    • Calpain 2 large subunit antibody
    • Calpain 2, large [catalytic] subunit antibody
    • Calpain large polypeptide L2 antibody
    • Calpain M type antibody
    • Calpain M-type antibody
    • Calpain-2 catalytic subunit antibody
    • Calpain-2 large subunit antibody
    • Calpain2 antibody
    • CAN2_HUMAN antibody
    • CANP 2 antibody
    • CANP L2 antibody
    • CANP2 antibody
    • CANPL 2 antibody
    • CANPL2 antibody
    • CANPml antibody
    • Capa2 antibody
    • CAPN 2 antibody
    • CAPN2 antibody
    • FLJ39928 antibody
    • M calpain antibody
    • M calpin antibody
    • M type antibody
    • M-calpain antibody
    • mCANP antibody
    • Millimolar calpain antibody
    • Millimolar-calpain antibody
    see all

Images

  • All lanes : Anti-Calpain 2 antibody [EPR5977] (ab126600) at 1/1000 dilution (Purified)

    Lane 1 : Rat brain lysates
    Lane 2 : Rat kidney lysates
    Lane 3 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : Mouse brain lysates
    Lane 5 : Mouse kidney lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 80 kDa
    Observed band size: 80 kDa

  • All lanes : Anti-Calpain 2 antibody [EPR5977] (ab126600) at 1/1000 dilution (unpurified)

    Lane 1 : PC3 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa

References

This product has been referenced in:
  • Zeng X  et al. Acylated and unacylated ghrelin inhibit atrophy in myotubes co-cultured with colon carcinoma cells. Oncotarget 8:72872-72885 (2017). Read more (PubMed: 29069832) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
(agent) for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (brain)
Specification
brain
Permeabilization
Yes - triton X-100
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Nov 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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