Recombinant
RabMAb

Anti-Calpain 2 antibody [EPR5977] (ab126600)

Overview

  • Product name
    Anti-Calpain 2 antibody [EPR5977]
    See all Calpain 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR5977] to Calpain 2
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region in the large subunit of Human Calpain 2.

  • Positive control
    • PC3, HeLa, A431 and 293T cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the peptidase C2 family.
      Contains 1 calpain catalytic domain.
      Contains 3 EF-hand domains.
    • Cellular localization
      Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calcium activated neutral proteinase 2 antibody
      • Calcium activated neutral proteinase antibody
      • Calcium-activated neutral protease 2, catalytic subunit antibody
      • Calcium-activated neutral proteinase 2 antibody
      • CALP80 antibody
      • Calpain 2 (m/II) large subunit antibody
      • Calpain 2 catalytic subunit antibody
      • Calpain 2 large catalytic subunit antibody
      • Calpain 2 large subunit antibody
      • Calpain 2, large [catalytic] subunit antibody
      • Calpain large polypeptide L2 antibody
      • Calpain M type antibody
      • Calpain M-type antibody
      • Calpain-2 catalytic subunit antibody
      • Calpain-2 large subunit antibody
      • Calpain2 antibody
      • CAN2_HUMAN antibody
      • CANP 2 antibody
      • CANP L2 antibody
      • CANP2 antibody
      • CANPL 2 antibody
      • CANPL2 antibody
      • CANPml antibody
      • Capa2 antibody
      • CAPN 2 antibody
      • CAPN2 antibody
      • FLJ39928 antibody
      • M calpain antibody
      • M calpin antibody
      • M type antibody
      • M-calpain antibody
      • mCANP antibody
      • Millimolar calpain antibody
      • Millimolar-calpain antibody
      see all

    Images

    • All lanes : Anti-Calpain 2 antibody [EPR5977] (ab126600) at 1/1000 dilution

      Lane 1 : PC3 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 80 kDa
      Observed band size : 80 kDa

    References

    ab126600 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    (agent) for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step
    None
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Permeabilization
    Yes - triton X-100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Nov 03 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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