Overview

  • Product nameAnti-Calpain 3 antibody
  • Description
    Rabbit polyclonal to Calpain 3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide corresponding to a region within C terminal amino acids 610-640 of Human Calpain 3.

  • Positive control
    • HL-60 cell lysate; Human skeletal muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab103250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 94 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionCalcium-regulated non-lysosomal thiol-protease.
  • Tissue specificityIsoform I is skeletal muscle specific.
  • Involvement in diseaseDefects in CAPN3 are the cause of limb-girdle muscular dystrophy type 2A (LGMD2A) [MIM:253600]. LGMD2A is an autosomal recessive degenerative myopathy characterized by progressive symmetrical atrophy and weakness of the proximal limb muscles and elevated serum creatine kinase. The symptoms usually begin during the first two decades of life, and the disease gradually worsens, often resulting in loss of walking ability 10 or 20 years after onset.
  • Sequence similaritiesBelongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 4 EF-hand domains.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium-activated neutral proteinase 3 antibody
    • calpain 3, (p94) antibody
    • Calpain L3 antibody
    • Calpain large polypeptide L3 antibody
    • Calpain p94 antibody
    • calpain p94, large [catalytic] subunit antibody
    • calpain, large polypeptide L3 antibody
    • Calpain-3 antibody
    • CAN3_HUMAN antibody
    • CANP 3 antibody
    • CANP3 antibody
    • CANPL3 antibody
    • CAPN3 antibody
    • LGMD 2A antibody
    • LGMD2 antibody
    • LGMD2A antibody
    • Lp82 antibody
    • Lp85 antibody
    • MGC10767 antibody
    • MGC11121 antibody
    • MGC14344 antibody
    • MGC4403 antibody
    • Muscle-specific calcium-activated neutral protease 3 antibody
    • muscle-specific calcium-activated neutral protease 3 large subunit antibody
    • nCL-1 antibody
    • New calpain 1 antibody
    • p94 antibody
    see all

Anti-Calpain 3 antibody images

  • Anti-Calpain 3 antibody (ab103250) at 1/50 dilution + HL-60 cell lysate at 35 µg

    Predicted band size : 94 kDa
  • ab103250, at 1/50 dilution, staining Calpain 3 in formalin-fixed, paraffin-embedded Human skeletal muscle by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.

References for Anti-Calpain 3 antibody (ab103250)

This product has been referenced in:
  • Kumchantuek T  et al. Expression and localization of calpain 3 in the submandibular gland of mice. Arch Oral Biol 70:9-15 (2016). WB ; Mouse . Read more (PubMed: 27295390) »

See 1 Publication for this product

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