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Storage instructions Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage buffer Preservative: 0.09% Sodium Azide
Concentration information loading...
Purity Ammonium Sulphate Precipitation
Purification notes ab103250 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Predicted molecular weight: 94 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Function Calcium-regulated non-lysosomal thiol-protease.
Tissue specificity Isoform I is skeletal muscle specific.
Involvement in disease Defects in CAPN3 are the cause of limb-girdle muscular dystrophy type 2A (LGMD2A) [MIM:253600]. LGMD2A is an autosomal recessive degenerative myopathy characterized by progressive symmetrical atrophy and weakness of the proximal limb muscles and elevated serum creatine kinase. The symptoms usually begin during the first two decades of life, and the disease gradually worsens, often resulting in loss of walking ability 10 or 20 years after onset.
Sequence similarities Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 4 EF-hand domains.
Cellular localization Cytoplasm.
Information by UniProt
Calcium-activated neutral proteinase 3 antibody
calpain 3, (p94) antibody
Calpain L3 antibody
Anti-Calpain 3 antibody images
Western blot - Calpain 3 antibody (ab103250)
Anti-Calpain 3 antibody (ab103250) at 1/50 dilution + HL-60 cell lysate at 35 µg Predicted band size : 94 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calpain 3 antibody (ab103250)
ab103250, at 1/50 dilution, staining Calpain 3 in formalin-fixed, paraffin-embedded Human skeletal muscle by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.
References for Anti-Calpain 3 antibody (ab103250)
has not yet been referenced specifically in any publications.
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