Calpain 3 antibody - Domain II (catalytic domain), large subunit (ab38962)

Product Name 

Calpain 3 antibody - Domain II (catalytic domain), large subunit

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 3 - Domain II (catalytic domain), large subunit

Immunogen 

Synthetic peptide based on Insert 1, in domain-II of human Calpain 3 large subunit.(Peptide available as ab41306.)

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat
(Due to sequence homology)

Specificity 

The antibody does not cross react with the other calpain family members (m-calpain, M-calpain, LP-82/85 Calpain, ncl-2, ncl-3, etc.). The antibody may crossreact with rat 88 and 90 kD novel Calpains due to Domain II sequence similarities.

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 3 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. When used against the reduced protein, Ab38962 identifies bands at 94 Kd, 82 Kd, 62 Kd, 60 Kd, and a series of further cleaved active forms. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain

Relevance 

Calpain 3, also known as calpain 94 or muscle calpain, is an intracellular, calcium dependent cysteine protease. The calpains have papain like activity, thus the pain nomenclature. The calpain family members consist of a common small subunit, and a large variable subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. Unlike M calpain which is ubiquitously expressed, Calpain 3 is a member of the tissue specific calpains. Unlike the other calpains, the endogenous calpain inhibitor, calpastatin, does not seem to inhibit Calpain 3. The half life of native Calpain 3 is also much shorter; about 10 minutes. Mutations in Calpain 3 have been linked to Limb Girdle Muscular Dystrophy, and native Calpain 3 is known to act as a meat tenderizer. The latent large subunit is 94 kDa, and the amino terminal truncation at activation yields an approximately 64 to 62 kDa form. A cascade of smaller forms can be seen with further activation.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Notes 

EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen (94 kDa form).

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