Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 3 antibody - Domain III, large subunit
See all Calpain 3 products (13) ...
Description
Rabbit polyclonal to Calpain 3 - Domain III, large subunit
Specificity
ab38963 recognises Domain III, (Insert 2), of Calpain 3 large subunit.This antibody does not cross react with the other calpain family members (m-calpain, M-calpain, LP-82/85 Calpain, ncl-2, ncl-3, etc.)
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide of Human Calpain 3 based on Insert 2, in domain-III of Calpain 3 large subunit. (Peptide available as ab41305.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. (A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.)
Target
Function
Calcium-regulated non-lysosomal thiol-protease.
Tissue specificity
Isoform I is skeletal muscle specific.
Involvement in disease
Defects in CAPN3 are the cause of limb-girdle muscular dystrophy type 2A (LGMD2A) [MIM:253600]. LGMD2A is an autosomal recessive degenerative myopathy characterized by progressive symmetrical atrophy and weakness of the proximal limb muscles and elevated serum creatine kinase. The symptoms usually begin during the first two decades of life, and the disease gradually worsens, often resulting in loss of walking ability 10 or 20 years after onset.
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP20807
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Calcium activated neutral proteinase 3 antibody
- Calcium-activated neutral proteinase 3 antibody
- Calpain L3 antibody
see all
Database links
- Entrez Gene: 825 Human
- Omim: 114240 Human
- SwissProt: P20807 Human
- Unigene: 143261 Human
Protocols
References for Anti-Calpain 3 antibody - Domain III, large subunit (ab38963)
This product has been referenced in:
- Moretti Det al. Novel variants of muscle calpain 3 identified in human melanoma cells: cisplatin-induced changes in vitro and differential expression in melanocytic lesions. Carcinogenesis 30:960-7 (2009).Read more (PubMed: 19386580) »
See 1 publication for this product
Publishing research using ab38963? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"