Overview

  • Product nameAnti-Calpain 5 antibody
    See all Calpain 5 primary antibodies
  • Description
    Rabbit polyclonal to Calpain 5
  • SpecificityDoes not cross react with the other calpain family members (Calpain 1, Calpain 2, Calpain 3, ncl 3, etc.).
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Calpain 5 aa 1-30 (N terminal).
    (Peptide available as ab41310)

  • General notesDiluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative.


    ab28280 does not recognize amino-processed Calpain 5, and can be used to discriminate between latent and amino-processed forms. ab28280 will also bind to the non-reduced protein, and thus may have application for immunoprecipitation, immunohistochemistry, and ELISA.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody has been peptide-affinity purified.
  • Primary antibody notesab28280 does not recognize amino-processed Calpain 5, and can be used to discriminate between latent and amino-processed forms. ab28280 will also bind to the non-reduced protein, and thus may have application for immunoprecipitation, immunohistochemistry, and ELISA.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab28280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 73, 68 kDa.

1/1000 (colorimetric) - 1/5000 (chemiluminescent). A series of further cleaved forms seen when used against the reduced protein. Dilution optimised using Chromogenic detection.

ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

Anti-Calpain 5 antibody images

  • ICC/IF image of ab28280 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28280, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Calpain 5 antibody (ab28280)

This product has been referenced in:
  • Singh R  et al. Calpain 5 is highly expressed in the central nervous system (CNS), carries dual nuclear localization signals, and is associated with nuclear promyelocytic leukemia protein bodies. J Biol Chem 289:19383-94 (2014). WB ; Rat, Human . Read more (PubMed: 24838245) »

See 1 Publication for this product

Product Wall

Thank you for your reply.

As this is a rabbit polyclonal antibody, we would expect some lot to lot variation in optimal antibody concentrations. What are the conditions of your current WB protocol? I would like to see if there are any sugges...

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Thank you for contacting Abcam and I am very sorry about the delay in getting you this information.
Answers to your questions:
1 - We used lysates from A2058 cells, and from RD cells in Western blot to look for Calpain-5 protein. We don't see...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"