Overview

  • Product name
  • Description
    Rabbit polyclonal to Calpain 5
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-351 of Human Calpain 5 (NP_004046)

  • Positive control
    • A431 and H1299 whole cell lysates; HeLa cells; 293T and HeLaS3 cell lines

Properties

Applications

Our Abpromise guarantee covers the use of ab97534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 73 kDa.
ICC/IF 1/100 - 1/200.

Target

Images

  • All lanes : Anti-Calpain 5 antibody (ab97534) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 73 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97534 at 1/100 dilution (top panel) and co-stained with Hoescht 33342 (bottom panel).

References

This product has been referenced in:
  • Schaefer KA  et al. Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses. Invest Ophthalmol Vis Sci 57:2509-21 (2016). Read more (PubMed: 27152965) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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