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Immunocytochemistry/ Immunofluorescence-Calpain 5 antibody - Aminoterminal end domain I(ab28280)
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Product Name
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Calpain 5 antibody - Aminoterminal end domain I
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See all Calpain 5 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Calpain 5 - Aminoterminal end domain I
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Immunogen
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Synthetic peptide based on the N terminal end of the large subunit of Human Calpain 5.(Peptide available as ab41310.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Does not cross react with the other calpain family members (Calpain 1, Calpain 2, Calpain 3, ncl 3, etc.).
We have a range of domain specific antibodies for this target. For a full list please see all Calpain 5 antibodies
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Epitope
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Reacts to the first 30 amino acids of the "propeptide domain," (Domain-I) of Calpain 5, N terminal from the activation cleavage site.
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
WB: 1/1000 (colorimetric) - 1/5000 (chemiluminescent). Detects bands of approximately 73 and 68 kDa and a series of further cleaved forms when used against the reduced protein. Dilution optimised using Chromogenic detection.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
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Relevance
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Calpain proteinases are defined currently as papain like neutral proteases that are calcium activated. Calpains 1 and 2 are composed of a large subunit, which is proteolytically active, and a small subunit (also called calpain 4), or enhancer protein, that is not proteolytically active. The other calpain proteins identified to date are not known to require a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV (Domain T in calpains 5 & 6).
Calpain 5, also known as the human orthologue of TRA3, a C. elegans protein involved in sex determination, is located on chromosome 11 in humans, near calpain 1. Calpain 5 is an intracellular, calcium dependent cysteine protease. Calpain 5 domain T does not have the standard calpain EF hand domain for binding calcium, making it unclear whether it is calcium regulated. It is also unclear if calpain 5 plays any role in mammalian sex determination, since the enzyme is found in colon, kidney, liver and trachea and other tissues, as well as testis.
ab28280 does not recognize amino-processed Calpain 5, and can be used to discriminate between latent and amino-processed forms. ab28280 will also bind to the non-reduced protein, and thus may have application for immunoprecipitation, immunohistochemistry, and ELISA.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody has been peptide-affinity purified.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calpain 5 antibody - Aminoterminal end domain I is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Calpain 5 antibody - Aminoterminal end domain I images, references, products related to ab28280 and other tools.
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Calpain 5 antibody - Aminoterminal end domain I images:
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 Immunocytochemistry/ Immunofluorescence-Calpain 5 antibody - Aminoterminal end domain I(ab28280)
ICC/IF image of ab28280 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28280, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to Calpain 5
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