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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 5 antibody - Carboxyterminal end domain IV
See all Calpain 5 products (5) ...
Description
Rabbit polyclonal to Calpain 5 - Carboxyterminal end domain IV
Specificity
The antibody binds to reduced and non-reduced Calpain 5, but does not cross react with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 2, ncl 3, etc.). It reacts to Domain-IV of Calpain 5, and detects both latent and aminoprocessed (activated) Calpain 5.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide of Human Calpain 5 based on the carboxyterminal end of the large subunit.
(Peptide available as ab44839.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000 - 1/5000. Detects bands of approximately 73 kDa (latent form) and 58 kDa (active form). (Predicted molecular weight: 73 kDa). Recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Calpain proteinases are defined currently as papain like neutral proteases that are calcium activated. Calpains 1 and 2 are composed of a large subunit, which is proteolytically active, and a small subunit (also called calpain 4), or enhancer protein, that is not proteolytically active. The other calpain proteins identified to date are not known to require a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV (Domain T in calpains 5 & 6). Calpain 5, also known as the human orthologue of TRA3, a C. elegans protein involved in sex determination, is located on chromosome 11 in humans, near calpain 1. Calpain 5 is an intracellular, calcium dependent cysteine protease. Calpain 5 domain T does not have the standard calpain EF hand domain for binding calcium, making it unclear whether it is calcium regulated. It is also unclear if calpain 5 plays any role in mammalian sex determination, since the enzyme is found in colon, kidney, liver and trachea and other tissues, as well as testis.
Cellular localization
Cytoplasmic
Alternative names
- CAPN 5 antibody
- CAPN5 antibody
- FLJ46245 antibody
see all
Database links
- Entrez Gene: 726 Human
- Entrez Gene: 12337 Mouse
- Entrez Gene: 171495 Rat
- Omim: 602537 Human
- SwissProt: O15484 Human
- SwissProt: O08688 Mouse
- SwissProt: Q3TDS7 Mouse
- SwissProt: Q3TPL4 Mouse
see all
Protocols
References for Anti-Calpain 5 antibody - Carboxyterminal end domain IV (ab28281)
ab28281 has not yet been referenced specifically in any publications.
Publishing research using ab28281? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"