Calpain 5 antibody - Carboxyterminal end domain IV (ab28281)

Product Name 

Calpain 5 antibody - Carboxyterminal end domain IV

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 5 - Carboxyterminal end domain IV

Immunogen 

Synthetic peptide of Human Calpain 5 based on the carboxyterminal end of the large subunit.

(Peptide available as ab44839.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

The antibody binds to reduced and non-reduced Calpain 5, but does not cross react with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 2, ncl 3, etc.). It reacts to Domain-IV of Calpain 5, and detects both latent and aminoprocessed (activated) Calpain 5.

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 5 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 - 1/5000. Detects bands of approximately 73 kDa (latent form) and 58 kDa (active form). (Predicted molecular weight: 73 kDa). Recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain

Relevance 

Calpain proteinases are defined currently as papain like neutral proteases that are calcium activated. Calpains 1 and 2 are composed of a large subunit, which is proteolytically active, and a small subunit (also called calpain 4), or enhancer protein, that is not proteolytically active. The other calpain proteins identified to date are not known to require a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV (Domain T in calpains 5 & 6).

Calpain 5, also known as the human orthologue of TRA3, a C. elegans protein involved in sex determination, is located on chromosome 11 in humans, near calpain 1. Calpain 5 is an intracellular, calcium dependent cysteine protease. Calpain 5 domain T does not have the standard calpain EF hand domain for binding calcium, making it unclear whether it is calcium regulated. It is also unclear if calpain 5 plays any role in mammalian sex determination, since the enzyme is found in colon, kidney, liver and trachea and other tissues, as well as testis.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Customer reviews (feedback) regarding Calpain 5 antibody - Carboxyterminal end domain IV

Customer FAQs regarding Calpain 5 antibody - Carboxyterminal end domain IV

Protocols for Calpain 5 antibody - Carboxyterminal end domain IV

Price and availability of products related to Calpain 5 antibody - Carboxyterminal end domain IV

Calpain 5 antibody - Carboxyterminal end domain IV - related products: