Anti-Calpain 6 antibody (ab76974)
Key features and details
- Rabbit polyclonal to Calpain 6
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Calpain 6 antibody -
Description
Rabbit polyclonal to Calpain 6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
An 18 amino acid peptide from near the carboxy terminus of human Calpain 6.
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Positive control
- WB: Rat lung tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 75 kDa.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 75 kDa. |
Target
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Function
Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues. -
Tissue specificity
Expressed only in placenta. -
Sequence similarities
Belongs to the peptidase C2 family.
Contains 1 C2 domain.
Contains 1 calpain catalytic domain. -
Cellular localization
Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > spindle. During mitose associated with the mitotic spindle. At telophase colocalized to the midbody spindle. - Information by UniProt
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Database links
- Entrez Gene: 83685 Rat
- SwissProt: O88501 Rat
- Unigene: 2862 Rat
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Alternative names
- CALP M antibody
- Calpain-6 antibody
- Calpain-like protease X-linked antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab76974 has been referenced in 2 publications.
- Zhang YY et al. Calpain 6 inhibits autophagy in inflammatory environments: A preliminary study on myoblasts and a chronic kidney disease rat model. Int J Mol Med 48:N/A (2021). PubMed: 34435644
- Filali H et al. Gene and protein patterns of potential prion-related markers in the central nervous system of clinical and preclinical infected sheep. Vet Res 44:14 (2013). IHC-P ; Sheep . PubMed: 23497022