Overview

  • Product nameAnti-Calpain 6 antibody
  • Description
    Rabbit polyclonal to Calpain 6
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    An 18 amino acid peptide from near the carboxy terminus of human Calpain 6.

  • Positive control
    • Rat lung tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab76974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 75 kDa.
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • FunctionMicrotubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues.
  • Tissue specificityExpressed only in placenta.
  • Sequence similaritiesBelongs to the peptidase C2 family.
    Contains 1 C2 domain.
    Contains 1 calpain catalytic domain.
  • Cellular localizationCytoplasm > perinuclear region. Cytoplasm > cytoskeleton > spindle. During mitose associated with the mitotic spindle. At telophase colocalized to the midbody spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • CALP M antibody
    • Calpain-6 antibody
    • Calpain-like protease X-linked antibody
    • Calpamodulin antibody
    • CalpM antibody
    • CAN6_HUMAN antibody
    • CANP X antibody
    • CANPX antibody
    • CAPN 6 antibody
    • Capn6 antibody
    see all

Anti-Calpain 6 antibody images

  • Lane 1 : Anti-Calpain 6 antibody (ab76974) at 0.5 µg/ml
    Lane 2 : Anti-Calpain 6 antibody (ab76974) at 1 µg/ml

    Lane 1 : rat lung tissue lysate
    Lane 2 : rat lung tissue lysate


    Predicted band size : 75 kDa
    Observed band size : 75 kDa
  • ab76974 at 2.5µg/ml staining Calpain6 in human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Immunofluorescence of CAPN6 in Human Lung cells using ab76974 at 20 ug/ml.

References for Anti-Calpain 6 antibody (ab76974)

This product has been referenced in:
  • Filali H  et al. Gene and protein patterns of potential prion-related markers in the central nervous system of clinical and preclinical infected sheep. Vet Res 44:14 (2013). IHC-P ; Sheep . Read more (PubMed: 23497022) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab76974.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"