Overview

  • Product name
    Anti-Calpain 6 antibody
  • Description
    Rabbit polyclonal to Calpain 6
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    An 18 amino acid peptide from near the carboxy terminus of human Calpain 6.

  • Positive control
    • Rat lung tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab76974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 75 kDa.
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Function
    Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues.
  • Tissue specificity
    Expressed only in placenta.
  • Sequence similarities
    Belongs to the peptidase C2 family.
    Contains 1 C2 domain.
    Contains 1 calpain catalytic domain.
  • Cellular localization
    Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > spindle. During mitose associated with the mitotic spindle. At telophase colocalized to the midbody spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • CALP M antibody
    • Calpain-6 antibody
    • Calpain-like protease X-linked antibody
    • Calpamodulin antibody
    • CalpM antibody
    • CAN6_HUMAN antibody
    • CANP X antibody
    • CANPX antibody
    • CAPN 6 antibody
    • Capn6 antibody
    see all

Images

  • Lane 1 : Anti-Calpain 6 antibody (ab76974) at 0.5 µg/ml
    Lane 2 : Anti-Calpain 6 antibody (ab76974) at 1 µg/ml

    Lane 1 : rat lung tissue lysate
    Lane 2 : rat lung tissue lysate


    Predicted band size : 75 kDa
    Observed band size : 75 kDa
  • ab76974 at 2.5µg/ml staining Calpain6 in human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Immunofluorescence of CAPN6 in Human Lung cells using ab76974 at 20 ug/ml.

References

This product has been referenced in:
  • Filali H  et al. Gene and protein patterns of potential prion-related markers in the central nervous system of clinical and preclinical infected sheep. Vet Res 44:14 (2013). IHC-P ; Sheep . Read more (PubMed: 23497022) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab76974.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up