Calpain 6 antibody - Domain II (catalytic domain), large subunit (ab38939)

Product Name 

Calpain 6 antibody - Domain II (catalytic domain), large subunit

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 6 - Domain II (catalytic domain), large subunit

Immunogen 

Synthetic peptide corresponding to the domain-II in the large subunit of Human Calpain 6.(Peptide available as ab41314.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab38939 binds to the Domain-II, large subunit of Calpain 6, but does not cross react with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 2, ncl 3, etc.).

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 6 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. When used against the reduced protein this antibody identifies bands at 75 kD, 58kD, and a series of further cleaved active forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain

Relevance 

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium- binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain 6 is highly expressed in the placenta. Its C-terminal region lacks any homology to the Calmodulin-like domain of other Calpains. It also lacks critical active site residues and thus is suggested to be proteolytically inactive.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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