Calpain 7 antibody - Aminoterminal end Domain I (ab28212)

Product Name 

Calpain 7 antibody - Aminoterminal end Domain I

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 7 - Aminoterminal end Domain I

Immunogen 

Synthetic peptide based on Aminoterminal Domain I of the large subunit of human calpain 7.(Peptide available as ab41318.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody does not cross react with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 3, etc).

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 7 antibodies

Epitope 

This antibody reacts to the Aminoterminal end Domain I of Calpain 7.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. When used against the reduced protein, ab28212 identifies bands at 93 Kd, 68 Kd, and a series of further cleaved forms. (predicted molecular weight: 93 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain

Relevance 

Calpain proteinases are defined currently as papain-like neutral proteases that are calcium activated. Calpains 1 and 2 are composed of a large subunit, which is proteolytically active, and a small subunit (also called calpain 4), or enhancer protein, that is not proteolytically active. The other calpain proteins identified to date (Calpain 5, 6, 8, 9, 10, 11, 12, 13, 14, 15) are not known to require a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV (domain-T in calpains 5 & 6, domain-N for calpain 7). Calpain 7 domain-N does not have the standard calpain EF hand domain for binding calcium, making it unclear whether it is calcium regulated. In place of the EF hand calcium-binding domain IV, Calpain 7 has a sequence most closely resembling domain-III, with unknown function. Calpain 7 is widely distributed, found in most tissues analyzed.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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