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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 7 antibody - Aminoterminal end Domain I
See all Calpain 7 products (5) ...
Description
Rabbit polyclonal to Calpain 7 - Aminoterminal end Domain I
Specificity
This antibody does not cross react with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 3, etc).
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide based on Aminoterminal Domain I of the large subunit of human calpain 7. (Peptide available as ab41318.)
Epitope
This antibody reacts to the Aminoterminal end Domain I of Calpain 7.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. When used against the reduced protein, ab28212 identifies bands at 93 Kd, 68 Kd, and a series of further cleaved forms. (predicted molecular weight: 93 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Calpain proteinases are defined currently as papain-like neutral proteases that are calcium activated. Calpains 1 and 2 are composed of a large subunit, which is proteolytically active, and a small subunit (also called calpain 4), or enhancer protein, that is not proteolytically active. The other calpain proteins identified to date (Calpain 5, 6, 8, 9, 10, 11, 12, 13, 14, 15) are not known to require a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV (domain-T in calpains 5 & 6, domain-N for calpain 7). Calpain 7 domain-N does not have the standard calpain EF hand domain for binding calcium, making it unclear whether it is calcium regulated. In place of the EF hand calcium-binding domain IV, Calpain 7 has a sequence most closely resembling domain-III, with unknown function. Calpain 7 is widely distributed, found in most tissues analyzed.
Cellular localization
Nuclear
Alternative names
- Calpain like protease antibody
- Calpain7 antibody
- CAPN 7 antibody
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Database links
- Entrez Gene: 23473 Human
- Entrez Gene: 12339 Mouse
- Entrez Gene: 306260 Rat
- Omim: 606400 Human
- SwissProt: Q7Z479 Human
- SwissProt: Q9Y6W3 Human
- SwissProt: Q501M2 Mouse
- SwissProt: Q9R1S8 Mouse
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Protocols
References for Anti-Calpain 7 antibody - Aminoterminal end Domain I (ab28212)
ab28212 has not yet been referenced specifically in any publications.
Publishing research using ab28212? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"