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Products:Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Overview
Product name
Anti-Calpain 7 antibody - Carboxyterminal end domain I
See all Calpain 7 products (5) ...
Description
Rabbit polyclonal to Calpain 7 - Carboxyterminal end domain I
Specificity
This antibody is specific for Calpain 7. It does not bind to other Calpain family members.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide based on Domain I of the large subunit, using the human Calpain 7 sequence. (Peptide available as ab41316.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 93kDa, 68kDa and a series of further cleaved forms. (predicted molecular weight: 93 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Calpain proteinases are defined currently as papain-like neutral proteases that are calcium activated. Calpains 1 and 2 are composed of a large subunit, which is proteolytically active, and a small subunit (also called calpain 4), or enhancer protein, that is not proteolytically active. The other calpain proteins identified to date (Calpain 5, 6, 8, 9, 10, 11, 12, 13, 14, 15) are not known to require a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV (domain-T in calpains 5 & 6, domain-N for calpain 7). Calpain 7 domain-N does not have the standard calpain EF hand domain for binding calcium, making it unclear whether it is calcium regulated. In place of the EF hand calcium-binding domain IV, Calpain 7 has a sequence most closely resembling domain-III, with unknown function. Calpain 7 is widely distributed, found in most tissues analyzed.
Cellular localization
Nuclear
Alternative names
- Calpain like protease antibody
- Calpain like protease antibody
- Calpain7 antibody
see all
Database links
- Entrez Gene: 23473 Human
- Entrez Gene: 12339 Mouse
- Entrez Gene: 306260 Rat
- Omim: 606400 Human
- SwissProt: Q7Z479 Human
- SwissProt: Q9Y6W3 Human
- SwissProt: Q501M2 Mouse
- SwissProt: Q9R1S8 Mouse
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Protocols
References for Anti-Calpain 7 antibody - Carboxyterminal end domain I (ab28211)
ab28211 has not yet been referenced specifically in any publications.
Publishing research using ab28211? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"