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Anti-Calpain 7 antibody - Carboxyterminal end domain-N (ab38937)

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Overview

Product name

Anti-Calpain 7 antibody - Carboxyterminal end domain-N
See all Calpain 7 products (5) ...

Description

Rabbit polyclonal to Calpain 7 - Carboxyterminal end domain-N

Specificity

ab38937 binds to Calpain 7, but does not cross react with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 3, etc.). It reacts to the Carboxyterminal end Domain-N of Calpain 7 and recognizes latent and amino-processed Calpain 7.

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to the Domain N of the large subunit of Human Calpain 7. (Peptide available as ab41317.)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain

  • Western blot - Calpain 7 antibody - Carboxyterminal end domain-N (ab38937)Western blot - Calpain 7 antibody - Carboxyterminal end domain-N (ab38937) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab38937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. When used against the reduced protein this antibody identifies bands at 93 Kd, 68 Kd, and a series of further cleaved forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Calpain proteinases are defined currently as papain-like neutral proteases that are calcium activated. Calpains 1 and 2 are composed of a large subunit, which is proteolytically active, and a small subunit (also called calpain 4), or enhancer protein, that is not proteolytically active. The other calpain proteins identified to date (Calpain 5, 6, 8, 9, 10, 11, 12, 13, 14, 15) are not known to require a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV (domain-T in calpains 5 & 6, domain-N for calpain 7). Calpain 7 domain-N does not have the standard calpain EF hand domain for binding calcium, making it unclear whether it is calcium regulated. In place of the EF hand calcium-binding domain IV, Calpain 7 has a sequence most closely resembling domain-III, with unknown function. Calpain 7 is widely distributed, found in most tissues analyzed.

Cellular localization

Nuclear

Alternative names

  • Calpain like protease antibody
  • Calpain7 antibody
  • CAPN 7 antibody
  • Homolog of Aspergillus Nidulans PALB antibody
  • PALBH antibody
see all

Database links

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Anti-Calpain 7 antibody - Carboxyterminal end domain-N images:

  Western blot - Calpain 7 antibody - Carboxyterminal end domain-N (ab38937)

Western blot - Calpain 7 antibody - Carboxyterminal end domain-N (ab38937)



Predicted band size : 93 kDa


Proteins were extracted from the various strata of the lens using a progressive lysis technique which, in this example, generated seven fractions, from the outer cell layers (F1) to the lens core (F7). Calpain 1, 2, and 7 are readily detected in the outer cell layers, but their abundance decreases with depth, and these calpains are not detected in the innermost (F7) fiber cells. In contrast, calpain 3 is not detected in the outermost cells, first appearing in the cortical (F2) fraction. Calpain 3 signal is maximal in F3-F4 and persists in the innermost (F7) fraction.

Calpain 1 : ab39170.

Calpain 2 : ab39165.

Calpain 3 : Non Abcam antibody.

Calpain 7 : ab38937.

Primary antibodies were detected using an horseradish peroxidase-conjugated secondary antibody and chemiluminescence.

Image from De Maria A et al, J Biol Chem. 2009 May 15;284(20):13542-50. Epub 2009 Mar 6, Fig 3. DOI 10.1074/jbc.M900561200

Protocols

  • Recommended WB protocol

    • References for Anti-Calpain 7 antibody - Carboxyterminal end domain-N (ab38937)

    This product has been referenced in:

    • De Maria Aet al. Calpain expression and activity during lens fiber cell differentiation. J Biol Chem 284:13542-50 (2009). WB; Mouse.Read more (PubMed: 19269960) »

    See 1 publication for this product

    Publishing research using ab38937? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"