Calpain 8 antibody - Domain III (ab28215)

Product Name 

Calpain 8 antibody - Domain III

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain 8 - Domain III

Immunogen 

Synthetic peptide (Rat) based on domain III of the large Calpain 8 subunit.(Peptide available as ab41354.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody binds to full length Calpain 8, but does not react with other calpain family members (Calpain 1, Calpain 2, Calpain 3, NCL-3, etc.)

We have a range of domain specific antibodies for this target. For a full list please see all Calpain 8 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Recommended starting dilution of 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for non human samples. Detects a band of approximately 80kDa, 60kDa and a series of cleaved forms, under reducing conditions. (Predicted molecular weight: 80 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Intracellular

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains

Signal Transduction >> Calcium Signaling >> Calpain

Relevance 

Calpain proteinases are defined currently as papain like neutral proteases that are calcium activated. Calpains 1 and 2 are composed of a large subunit, which is proteolytically active, and a small subunit (also called calpain 4), or enhancer protein, that is not proteolytically active. The other calpain proteins identified to date are not known to require a small subunit, although there is evidence that the calpain small subunit #2 may act as a chaperonin in the folding of calpains, and then disassociate. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV (Domain T in calpains 5 & 6).

Calpain 8, also known as NCL 2 (novel calpain 2), was originally characterized in rodents as a stomach specific protein, but the human sequence was isolated from leukocytes. Most similar to calpain 2 (60 % identical to rat or human sequences), murine calpain 8 is also known as a truncated form (Calpain NCL-2), lacking the calcium binding domain, and most of domain III. Homology between human calpain 8 and human calpain 9 (the human stomach specific calpain) is much lower; calpain 9 is more similar to calpain 3. Homology between rat, mouse and human calpain 8 is high. The large subunit of calpain 8 zymogen runs at approximately 80 Kd, and the amino terminal truncation at activation yields an approximately 60 kD form. Cleavage of the carboxyterminal region generates smaller forms of Calpain 8, but it is not clear if these forms are proteolytically active.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody has been peptide affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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