You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-Calpain 9 antibody - Domain IV (EF-1), large subunit (ab28218)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-Calpain 9 antibody - Domain IV (EF-1), large subunit
See all Calpain 9 products (8) ...

Description

Rabbit polyclonal to Calpain 9 - Domain IV (EF-1), large subunit

Specificity

This antibody recognizes latent and aminotruncated (active) calpain 9, but not the truncated form lacking the domain-IV. It does not cross react with the other calpain family members (m-calpain, calpain-94, ncl-2, ncl-3, etc.)

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide (Human) based on domain-IV in the large subunit. (Peptide available as ab41358.)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

Application notes

WB: 1/1000 - 1/5000. Detects a band of approximately 80 kDa and a series of bands due to cleaved forms, (under reducing conditions). (predicted molecular weight: 79 kDa). Notes: a recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Calcium-regulated non-lysosomal thiol-protease.

Tissue specificity

Expressed predominantly in stomach.

Sequence similarities

Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains.

Target information above from: UniProt accessionO14815 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Calpain-9 antibody
  • Calpain9 antibody
  • CAN9_HUMAN antibody
  • CAPN 9 antibody
  • CAPN9 antibody
  • CG36 protein antibody
  • Digestive tract specific calpain antibody
  • Digestive tract-specific calpain antibody
  • GC36 antibody
  • nCL-4 antibody
  • nCL4 antibody
  • New calpain 4 antibody
  • novel calpain large subunit-4 antibody
  • novel calpain large subunit4 antibody
  • OTTHUMP00000035899 antibody
  • OTTHUMP00000035900 antibody
  • Protein CG36 antibody
see all

Database links

see all

Protocols

  • Recommended WB protocol

    • References for Anti-Calpain 9 antibody - Domain IV (EF-1), large subunit (ab28218)

    ab28218 has not yet been referenced specifically in any publications.

    Publishing research using ab28218? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"