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Rabbit monoclonal [EPR13422] to Calpain 9
Recombinant fragment within Human Calpain 9 aa 300-450. The exact sequence is proprietary.
Database link: O14815
Human stomach lysate; HepG2 cells.
This product is a recombinant rabbit monoclonal antibody.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250.
1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 79 kDa).
Calcium-regulated non-lysosomal thiol-protease.
Expressed predominantly in stomach.
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain. Contains 3 EF-hand domains.
Information by UniProt
Western blot - Anti-Calpain 9 [EPR13422] antibody (ab181178)
Anti-Calpain 9 antibody [EPR13422] (ab181178) at 1/10000 dilution + Human stomach lysate at 20 µg
Secondary goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution Developed using the ECL technique Predicted band size : 79 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Calpain 9 [EPR13422] antibody (ab181178)
Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling Calpain 9 with ab181178 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"