Recombinant
RabMAb

Anti-Calpain 9 antibody [EPR13422] (ab181178)

Overview

  • Product name
    Anti-Calpain 9 antibody [EPR13422]
    See all Calpain 9 primary antibodies
  • Description
    Rabbit monoclonal [EPR13422] to Calpain 9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Calpain 9 aa 300-450. The exact sequence is proprietary.
    Database link: O14815

  • Positive control
    • Human stomach lysate; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 79 kDa).

Target

  • Function
    Calcium-regulated non-lysosomal thiol-protease.
  • Tissue specificity
    Expressed predominantly in stomach.
  • Sequence similarities
    Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 3 EF-hand domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calpain-9 antibody
    • Calpain9 antibody
    • CAN9_HUMAN antibody
    • CAPN 9 antibody
    • CAPN9 antibody
    • CG36 protein antibody
    • Digestive tract specific calpain antibody
    • Digestive tract-specific calpain antibody
    • GC36 antibody
    • nCL-4 antibody
    • nCL4 antibody
    • New calpain 4 antibody
    • novel calpain large subunit-4 antibody
    • novel calpain large subunit4 antibody
    • OTTHUMP00000035899 antibody
    • OTTHUMP00000035900 antibody
    • Protein CG36 antibody
    see all

Images

  • Anti-Calpain 9 antibody [EPR13422] (ab181178) at 1/10000 dilution + Human stomach lysate at 20 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 79 kDa

  • Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling Calpain 9 with ab181178 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

References

ab181178 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab181178.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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