Overview

  • Product name
  • Description
    Rabbit polyclonal to Calpain S1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-222 of Human Calpain S1 (UniProt ID: P04632).

  • Positive control
    • 293T whole cell lysate; HeLa cells; Human lung adenocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Sequence similarities
    Contains 5 EF-hand domains.
  • Domain
    The contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer.
    EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant.
  • Cellular localization
    Cytoplasm. Cell membrane. Translocates to the plasma membrane upon calcium binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • 30K antibody
    • Calcium activated neutral proteinase antibody
    • Calcium activated neutral proteinase small subunit antibody
    • Calcium dependent protease small subunit 1 antibody
    • Calcium dependent protease small subunit antibody
    • Calcium-activated neutral proteinase small subunit antibody
    • Calcium-dependent protease small subunit 1 antibody
    • Calcium-dependent protease small subunit antibody
    • Calpain 4 antibody
    • Calpain 4 small subunit (30K) antibody
    • Calpain 4 small subunit antibody
    • Calpain regulatory subunit antibody
    • Calpain small polypeptide antibody
    • Calpain small subunit 1 antibody
    • Calpain4 antibody
    • CalpainS1 antibody
    • CANP antibody
    • CANP small subunit antibody
    • CANPS antibody
    • CAPN 4 antibody
    • CAPN S1 antibody
    • CAPN4 antibody
    • CAPNS 1 antibody
    • CAPNS antibody
    • Capns1 antibody
    • CDPS antibody
    • CPNS1_HUMAN antibody
    • CSS 1 antibody
    • CSS1 antibody
    see all

Images

  • Anti-Calpain S1 antibody (ab155672) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 28 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells (iced-cold; 5 mins) labeling CAPNS1 with ab155672 at 1/500 dilution (green). Right image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue, labeling Calpain S1 with ab155672 at 1/100 dilution.

References

ab155672 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155672.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up