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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-Calpain S1 antibody - Aminoterminal end domain V
See all Calpain S1 products (13) ...
Description
Rabbit polyclonal to Calpain S1 - Aminoterminal end domain V
Specificity
ab28237 binds to calpain S-1, does not cross react with the other calpain family members (calpain S-2, calpain-1, calpain-2, calpain-3, etc.).
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide based on the amino terminal end of domain-V of the small subunit of Human Calpain S1.
Positive control
mouse brain tissue lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Signal Transduction >> Metabolism >> Amino Acids
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Immunocytochemistry/ Immunofluorescence - Calpain S1 antibody - Aminoterminal end domain V (ab28237)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calpain S1 antibody - Aminoterminal end domain V (ab28237)
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Western blot - Calpain S1 antibody - Aminoterminal end domain V (ab28237)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab28237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/1000 when using colorimetric substrates - 1/5000 for chemiluminescent substrates. Predicted molecular weight: 30 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Calpain S1 is a small subunit common to both Calpain I and II and is associated with myotonic dystrophy.
Cellular localization
Cytoplasmic; translocates to the plasma membrane upon calcium binding
Alternative names
- 30K antibody
- Calcium activated neutral proteinase antibody
- Calcium Activated Neutral Proteinase Small Subunit antibody
see all
Database links
- Entrez Gene: 826 Human
- Entrez Gene: 12336 Mouse
- Entrez Gene: 29156 Rat
- Omim: 114170 Human
- SwissProt: P04632 Human
- SwissProt: O88456 Mouse
- SwissProt: Q64537 Rat
- Unigene: 515371 Human
see all
Anti-Calpain S1 antibody - Aminoterminal end domain V images:
Immunocytochemistry/ Immunofluorescence - Calpain S1 antibody - Aminoterminal end domain V (ab28237)
ICC/IF image of ab28237 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28237, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calpain S1 antibody - Aminoterminal end domain V (ab28237)
Ab28237 staining Human normal renal medulla. Staining is localized the nucleus and cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Western blot - Calpain S1 antibody - Aminoterminal end domain V (ab28237)
Predicted band size : 30 kDa
Plasma membrane (Membrane) and cytoplasmic (Cytosol) fractions were prepared from HUVEC cells and immunoblotted with antibodies against the indicated proteins. HRP-conjugated secondary antibodies were used. Blots are representative of four independent experiments.
Image from Su SC et al, J Biol Chem. 2010 Dec 24;285(52):40624-34. Epub 2010 Oct 13, Fig 2.
Protocols
References for Anti-Calpain S1 antibody - Aminoterminal end domain V (ab28237)
This product has been referenced in:
- McHugh BJet al. Integrin activation by Fam38A uses a novel mechanism of R-Ras targeting to the endoplasmic reticulum. J Cell Sci 123:51-61 (2010). WB; Human.Read more (PubMed: 20016066) »
- Su SCet al. Annexin 2 Regulates Endothelial Morphogenesis by Controlling AKT Activation and Junctional Integrity. J Biol Chem 285:40624-34 (2010). WB; Human.Read more (PubMed: 20947498) »
See all 3 publications for this product
Publishing research using ab28237? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"