| | | | Immunocytochemistry/ Immunofluorescence - Calpain S1 antibody - Aminoterminal end domain V (ab28237)
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| Product Name | Calpain S1 antibody - Aminoterminal end domain V |
| | See all Calpain S1 antibodies (10)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to Calpain S1 - Aminoterminal end domain V |
| Immunogen | Synthetic peptide based on the amino terminal end of domain-V of the small subunit of Human Calpain S1.
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| Reacts with
(species key) | Hu, Ms, Rat |
| Specificity | ab28237 binds to calpain S-1, does not cross react with the other calpain family members (calpain S-2, calpain-1, calpain-2, calpain-3, etc.). We have a range of domain specific antibodies for this target. For a full list please see all Calpain S1 antibodies |
| Tested applications
(see key) | ICC/IF, WB |
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| Application notes
(see key) | Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 when using colorimetric substrates - 1/5000 for chemiluminescent substrates. Predicted molecular weight: 30 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Positive control
(see definition) | mouse brain tissue lysate |
| Cellular localization | Cytoplasmic; translocates to the plasma membrane upon calcium binding |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain
Signal Transduction >> Metabolism >> Amino Acids |
| Relevance | Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Calpain S1 is a small subunit common to both Calpain I and II and is associated with myotonic dystrophy. |
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| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calpain S1 antibody - Aminoterminal end domain V is on this datasheet. But please do contact us if you would like any reassurance! |
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| See below for Calpain S1 antibody - Aminoterminal end domain V images, references, products related to ab28237 and other tools. |
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Calpain S1 antibody - Aminoterminal end domain V images: |
 Immunocytochemistry/ Immunofluorescence - Calpain S1 antibody - Aminoterminal end domain V (ab28237)
ICC/IF image of ab28237 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28237, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. |
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| Search PubMed (MEDLINE) for references to Calpain S1 |
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