Calpain S1 antibody - Carboxyterminal end domain V (ab28217)

Product Name 

Calpain S1 antibody - Carboxyterminal end domain V

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain S1 - Carboxyterminal end domain V

Immunogen 

Synthetic peptide (Human) based on the carboxyterminal end of domain V of the small subunit.(Peptide available as ab41356.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody binds to calpain S1, and recognizes latent and amino processed forms. It does not cross react with the other calpain family members (calpain S2, calpain 1, calpain 2, calpain 3, etc.)

We have a range of domain specific antibodies for this target. For a full list please see all Calpain S1 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Recommended starting dilution of 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects bands of approximately 30kDa and 28kDa (amino truncated form) under reducing conditions. (Predicted molecular weight: 30 kDa). Calpain S1 is quickly degraded, hence samples must be lysed in the presence of protease inhibiters to maintain latency. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic; translocates to the plasma membrane upon calcium binding

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain
Signal Transduction >> Metabolism >> Amino Acids

Relevance 

Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Calpain S1 is a small subunit common to both Calpain I and II and is associated with myotonic dystrophy.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody has been peptide affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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