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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-Calpain S1 antibody - Carboxyterminal end domain V
See all Calpain S1 products (13) ...
Description
Rabbit polyclonal to Calpain S1 - Carboxyterminal end domain V
Specificity
This antibody is specific for Calpain S1. It does not cross react with the other calpain family members(calpain S-2, calpain-1, calpain-2, calpain-3, etc.).
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide based on the carboxyterminal end of domain-V of the small subunit, using the human Calpain S1 sequence.
(Peptide available as ab41376.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calpain
Signal Transduction >> Metabolism >> Amino Acids
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Calpain S1 antibody - Carboxyterminal end domain V(ab28238)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab28238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
- 1 Image
IHC-P
Application notes
IHC-P: Use at a concentration of 1 µg/ml.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 30kDa and 28kDa (predicted molecular weight: 30 kDa). Calpain S1 is quickly degraded, and samples must be lysed in the presence of protease inhibiters to maintain latency. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Calpain S1 is a small subunit common to both Calpain I and II and is associated with myotonic dystrophy.
Cellular localization
Cytoplasmic; translocates to the plasma membrane upon calcium binding
Alternative names
- 30K antibody
- Calcium activated neutral proteinase antibody
- Calcium Activated Neutral Proteinase Small Subunit antibody
see all
Database links
- Entrez Gene: 826 Human
- Entrez Gene: 12336 Mouse
- Entrez Gene: 29156 Rat
- Omim: 114170 Human
- SwissProt: P04632 Human
- SwissProt: O88456 Mouse
- SwissProt: Q64537 Rat
- Unigene: 515371 Human
see all
Anti-Calpain S1 antibody - Carboxyterminal end domain V images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Calpain S1 antibody - Carboxyterminal end domain V(ab28238)
Ab28238 staining human normal skeletal muscle. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
References for Anti-Calpain S1 antibody - Carboxyterminal end domain V (ab28238)
ab28238 has not yet been referenced specifically in any publications.
Publishing research using ab28238? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"