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| Product Name | Calpain S1 antibody - Carboxyterminal end domain VI |
| | See all Calpain S1 antibodies (10)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to Calpain S1 - Carboxyterminal end domain VI |
| Immunogen | Synthetic peptide based on the carboxyterminal end of domain-VI of the small subunit, using the human Calpain S1 sequence. |
| Reacts with
(species key) | Hu, Ms, Rat |
| Specificity | This antibody is specific for Calpain S1. It does not cross react with the other calpain family members (calpain S-2, calpain-1, calpain-2, calpain-3, etc...) We have a range of domain specific antibodies for this target. For a full list please see all Calpain S1 antibodies |
| Tested applications
(see key) | WB |
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| Abreviews | |
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| Application notes
(see key) | Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 30kDa and 28kDa (predicted molecular weight: 30 kDa). Calpain S1 is quickly degraded, and samples must be lysed in the presence of protease inhibiters to maintain latency. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Cellular localization | Cytoplasmic; translocates to the plasma membrane upon calcium binding |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain
Signal Transduction >> Metabolism >> Amino Acids |
| Relevance | Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Calpain S1 is a small subunit common to both Calpain I and II and is associated with myotonic dystrophy. |
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| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calpain S1 antibody - Carboxyterminal end domain VI is on this datasheet. But please do contact us if you would like any reassurance! |
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| Search PubMed (MEDLINE) for references to Calpain S1 |
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