Calpain S2 antibody - Carboxyterminal end domain VI (ab38946)

Product Name 

Calpain S2 antibody - Carboxyterminal end domain VI

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calpain S2 - Carboxyterminal end domain VI

Immunogen 

Synthetic peptide based on the carboxyterminal end of domain-VI of the small subunit of Human Calpain S2.

(Peptide available as ab41380.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab38946 binds to calpain S2, but does not cross react with the other calpain family members (calpain S1, calpain 1, calpain 2, calpain 3, etc.). ab38946 recognizes latent and amino-processed calpain S1; the latent small subunit is 30 Kd, and the amino terminal truncation at activation yields an approximately 28 kD form.

We have a range of domain specific antibodies for this target. For a full list please see all Calpain S2 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. When used against the reduced protein this antibody identifies bands at 28 kD and 26 kD. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic; Translocates to the plasma membrane upon calcium binding

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Signal Transduction >> Calcium Signaling >> Calpain

Relevance 

Both M-calpain and m-calpain are ubiquitously expressed in mammalian cells, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain-3, calpain-5, calpain-9, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain-S1), and a large variable subunit. It is not clear that all calpains form heterodimers with a small subunit, but calpain-S1 is known to form a heterodimer with calpain-1 and also with calpain-2. Calpain S2 was recently discovered, and initial studies indicate that both small subunits may act as chaperonins to help with folding, or accessory proteins to help localize the calpains. Domains in the small subunit include the amino terminal domain-V (a glycine-rich sequence that is removed on activation of calpain, shorter in calpain-S2 than in calpain S1) and domain-VI, the calcium binding, EF hand domain.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab38946 has been peptide-affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Notes 

Store diluted antibody at 4°C with 0.05% Sodium Azide as preservative.

Customer reviews (feedback) regarding Calpain S2 antibody - Carboxyterminal end domain VI

Customer FAQs regarding Calpain S2 antibody - Carboxyterminal end domain VI

Protocols for Calpain S2 antibody - Carboxyterminal end domain VI

Price and availability of products related to Calpain S2 antibody - Carboxyterminal end domain VI

Calpain S2 antibody - Carboxyterminal end domain VI - related products: