Overview

  • Product nameAnti-Calpastatin antibody
    See all Calpastatin primary antibodies
  • Description
    Mouse monoclonal to Calpastatin
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: QPDPDENKPM EDKVKEKAKA EHRDKLGERD DTIPPEYRHL LDDNGQDKPV, corresponding to amino acids 582-670 (NP_001741) of Human Calpastatin with proprietary tag.

  • Positive control
    • NIH 3T3 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab88065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 76 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionSpecific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
  • Sequence similaritiesBelongs to the protease inhibitor I27 (calpastatin) family.
  • DomainHas four inhibitory domains.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Information by UniProt
  • Database links
  • Alternative names
    • BS 17 antibody
    • Calpain inhibitor antibody
    • Calpastatin antibody
    • Cast antibody
    • Heart type calpastatin antibody
    • ICAL_HUMAN antibody
    • MGC9402 antibody
    • Sperm BS 17 component antibody
    • Sperm BS-17 component antibody
    see all

Anti-Calpastatin antibody images

  • Anti-Calpastatin antibody (ab88065) at 5 µg/ml + recombinant protein used as immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 76 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-Calpastatin antibody (ab88065) at 5 µg/ml + NIH 3T3 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 76 kDa
    Observed band size : 76 kDa

References for Anti-Calpastatin antibody (ab88065)

ab88065 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"