The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 76 kDa.
Use at an assay dependent dilution.
FunctionSpecific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Sequence similaritiesBelongs to the protease inhibitor I27 (calpastatin) family.
DomainHas four inhibitory domains.
Post-translational modificationsThe N-terminus is blocked.