| | | | Immunocytochemistry/ Immunofluorescence-Calpastatin antibody - Carboxyterminal end long isoform(ab28253)
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| Product Name | Calpastatin antibody - Carboxyterminal end long isoform |
| | See all Calpastatin antibodies (9)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to Calpastatin - Carboxyterminal end long isoform |
| Immunogen | Synthetic peptide to the carboxyterminal end of human Calpastatin long isoform.
(Peptide available as ab41381.) |
| Reacts with
(species key) | Hu, Ms, Rat |
| Specificity | ab28253 recognises the C terminal domain of Calpastatin long isoform. We have a range of domain specific antibodies for this target. For a full list please see all Calpastatin antibodies |
| Tested applications
(see key) | ICC/IF, WB |
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| Application notes
(see key) | Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 76 kDa. But note that when used against the reduced protein, ab28253 identifies bands at 110 Kd, 82 Kd, and a series of further cleaved active forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Cellular localization | Cytoplasmic and Nuclear |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Calpains
Neuroscience >> Neuronal Markers >> Neuron >> Synapse
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurodegeneration >> Alzheimer's >> Other |
| Relevance | Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. |
| | Alternative names for Calpastatin antibody - Carboxyterminal end long isoform (ab28253) |
| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Calpastatin antibody - Carboxyterminal end long isoform is on this datasheet. But please do contact us if you would like any reassurance! |
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| See below for Calpastatin antibody - Carboxyterminal end long isoform images, references, products related to ab28253 and other tools. |
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Calpastatin antibody - Carboxyterminal end long isoform images: |
 Immunocytochemistry/ Immunofluorescence-Calpastatin antibody - Carboxyterminal end long isoform(ab28253)
ICC/IF image of ab28253 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28253, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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| Search PubMed (MEDLINE) for references to Calpastatin |
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