Overview

  • Product name
    Anti-Calpastatin antibody [SP82]
    See all Calpastatin primary antibodies
  • Description
    Rabbit monoclonal [SP82] to Calpastatin
  • Tested applications
    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide derived from near the C-terminus of Human Calpastatin protein.

  • Positive control
    • Human breast carcinoma

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab101684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Incubate for 30 minutes at RT. Antigen retrieval is recommended: boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
  • Sequence similarities
    Belongs to the protease inhibitor I27 (calpastatin) family.
  • Domain
    Has four inhibitory domains.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Information by UniProt
  • Database links
  • Alternative names
    • BS 17 antibody
    • Calpain inhibitor antibody
    • Calpastatin antibody
    • Cast antibody
    • Heart type calpastatin antibody
    • ICAL_HUMAN antibody
    • MGC9402 antibody
    • Sperm BS 17 component antibody
    • Sperm BS-17 component antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab101684 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab101684, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunohistochemical analysis of Calpastatin in formalin-fixed, paraffin-embedded Human breast carcinoma using ab101684 at 1/200 dilution.

References

ab101684 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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