Calpastatin overexpression 293T lysate (whole cell) (ab94278)

Overview

  • Product nameCalpastatin overexpression 293T lysate (whole cell)
    See all Calpastatin lysates
  • Description
    Calpastatin overexpression lysate
  • General notesab94278 is a 293T cell transfected lysate in which Human Calpastatin has been transiently over-expressed using a pCMV-Calpastatin plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. Similarity: Belongs to the protease inhibitor I27 (calpastatin) family. Domain: Has four inhibitory domains. PTM: The N-terminus is blocked.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Calpastatin overexpression 293T lysate (whole cell) images

  • ab94278 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Calpastatin antibody (ab88065) at 1/500 dilution

    Lane 1 : Calpastatin overexpression 293T lysate (whole cell) (ab94278)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Calpastatin overexpression 293T lysate (whole cell) (ab94278)

ab94278 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"