Overview

  • Product name
    Anti-Calponin antibody [26A11]
    See all Calponin primary antibodies
  • Description
    Mouse monoclonal [26A11] to Calponin
  • Specificity
    Western blotting data indicates that this antibody recognises the 34kD basic form of Calponin and not the 29kD variant.
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Prokaryotic recombinant protein corresponding to 75 amino acids of the C terminal of the basic Calponin molecule.

  • Positive control
    • Bowel, appendix, smooth muscle and SAOS 2 cell line.
  • General notes
    Staining pattern; Cytoplasmic.

Properties

Applications

Our Abpromise guarantee covers the use of ab50842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/50 - 1/100. Predicted molecular weight: 33 kDa.
IHC-Fr 1/25 - 1/50. Fix with acetone.

Target

  • Function
    Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
  • Tissue specificity
    Smooth muscle, and tissues containing significant amounts of smooth muscle.
  • Sequence similarities
    Belongs to the calponin family.
    Contains 3 calponin-like repeats.
    Contains 1 CH (calponin-homology) domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic calponin antibody
    • Calponin 1 antibody
    • Calponin 1 basic smooth muscle antibody
    • Calponin H1 antibody
    • Calponin H1 smooth muscle antibody
    • Calponin-1 antibody
    • Calponin1 antibody
    • Calponins basic antibody
    • CNN 1 antibody
    • Cnn1 antibody
    • CNN1_HUMAN antibody
    • Epididymis secretory protein Li 14 antibody
    • HEL S 14 antibody
    • Sm Calp antibody
    • SMCC antibody
    • smooth muscle antibody
    see all

Images

  • Immunohistochemical staining of basic Calponin in normal human appendix, using ab50842 at a 1/25 dilution. Note cytoplasmic staining of smooth muscle.

References

ab50842 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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