The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/50 - 1/100. Predicted molecular weight: 33 kDa.
1/25 - 1/50. Fix with acetone.
FunctionThin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Tissue specificitySmooth muscle, and tissues containing significant amounts of smooth muscle.
Sequence similaritiesBelongs to the calponin family. Contains 3 calponin-like repeats. Contains 1 CH (calponin-homology) domain.