The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionThin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Tissue specificitySmooth muscle, and tissues containing significant amounts of smooth muscle.
Sequence similaritiesBelongs to the calponin family. Contains 3 calponin-like repeats. Contains 1 CH (calponin-homology) domain.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human breast tissue labelled with ab110128 at 3 µg/ml. Followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human prostate tissue labelled with ab110128 at 3 µg/ml. Followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-Calponin antibody (ab110128)
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