Anti-Calponin 1 antibody (ab78491)
Key features and details
- Rabbit polyclonal to Calponin 1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Calponin 1 antibody
See all Calponin 1 primary antibodies -
Description
Rabbit polyclonal to Calponin 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Calponin 1 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab90216) -
Positive control
- Recombinant Human Calponin protein (ab114213) can be used as a positive control in WB. This antibody gave a positive signal in the following Human tissue lysates: Small Intestine; Heart; Bladder; Colon.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).
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Notes |
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IHC-P
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa). |
Target
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Function
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. -
Tissue specificity
Smooth muscle, and tissues containing significant amounts of smooth muscle. -
Sequence similarities
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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Database links
- Entrez Gene: 1264 Human
- Omim: 600806 Human
- SwissProt: P51911 Human
- Unigene: 465929 Human
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Alternative names
- Basic calponin antibody
- Calponin 1 antibody
- Calponin 1 basic smooth muscle antibody
see all
Images
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All lanes : Anti-Calponin 1 antibody (ab78491) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Human bladder tissue lysate - soluble fraction (female, 45 years) (ab44654)
Lane 4 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes -
IHC image of ab78491 staining in Human Normal Breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78491, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab78491 has been referenced in 1 publication.
- Zhang Q et al. MiR-30c-5p regulates adventitial progenitor cells differentiation to vascular smooth muscle cells through targeting OPG. Stem Cell Res Ther 12:67 (2021). PubMed: 33468212