The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This antibody is pre-diluted ready for use with an ABC system.
For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval before commencing with IHC.
Calponin has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast.
Calponin has also been useful in malignant myoepithelium and pleomorphoric adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions.
FunctionThin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Tissue specificitySmooth muscle, and tissues containing significant amounts of smooth muscle.
Sequence similaritiesBelongs to the calponin family. Contains 3 calponin-like repeats. Contains 1 CH (calponin-homology) domain.