Anti-Calreticulin 3 antibody (ab57346)


  • Product nameAnti-Calreticulin 3 antibody
    See all Calreticulin 3 primary antibodies
  • Description
    Mouse monoclonal to Calreticulin 3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 21-385 of Human Calreticulin 3



Our Abpromise guarantee covers the use of ab57346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDuring spermatogenesis, may act as a lectin-independent chaperone for specific client proteins such as ADAM3. Required for sperm fertility (By similarity). CALR3 capacity for calcium-binding may be absent or much lower than that of CALR.
    • Tissue specificityTestis specific.
    • Involvement in diseaseDefects in CALR3 are the cause of familial hypertrophic cardiomyopathy type 19 (CMH19) [MIM:613875]. CMH19 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    • Sequence similaritiesBelongs to the calreticulin family.
    • DomainCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
      The interaction with glycans occurs through a binding site in the globular lectin domain.
      The zinc binding sites are localized to the N-domain.
    • Cellular localizationEndoplasmic reticulum lumen.
    • Information by UniProt
    • Database links
    • Alternative names
      • CALR 3 antibody
      • CALR3 antibody
      • CALR3_HUMAN antibody
      • Calreticulin 2 antibody
      • Calreticulin-2 antibody
      • Calreticulin-3 antibody
      • Calreticulin2 antibody
      • Calreticulin3 antibody
      • Calsperin antibody
      • Cancer/testis antigen 93 antibody
      • CMH19 antibody
      • CRT2 antibody
      • CT93 antibody
      • FLJ25355 antibody
      • MGC26577 antibody
      see all

    Anti-Calreticulin 3 antibody images

    • Western blot against tagged recombinant protein immunogen using ab57346 Calreticulin 3 antibody at 1ug/ml. Predicted band size of immunogen is 66 kDa

    References for Anti-Calreticulin 3 antibody (ab57346)

    ab57346 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HUVEC)
    Loading amount 27 µg
    Specification HUVEC
    Gel Running Conditions Non-reduced Denaturing (7.5% Acrylamide)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Dr. Anna Armoza

    Verified customer

    Submitted Apr 12 2011