Overview

  • Product nameAnti-Calreticulin antibody
    See all Calreticulin primary antibodies
  • Description
    Sheep polyclonal to Calreticulin
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Carboxyl terminal domain of human calreticulin.

  • Positive control
    • MCF-7 cells
  • General notes


    This antibody can be used as a marker for subcellular fractionation of cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis antibody is provided as a 0.2µm sterile filtered solution.
  • Primary antibody notesThis antibody can be used as a marker for subcellular fractionation of cells.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 48 kDa.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionMolecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • Sequence similaritiesBelongs to the calreticulin family.
  • DomainCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • Cellular localizationEndoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all

Anti-Calreticulin antibody images

  • All lanes : Anti-Calreticulin antibody (ab16144) at 1/5000 dilution

    Lane 1 : Whole cell lysate prepared from Human Jurkat cells at 2 µg
    Lane 2 : Whole cell lysate prepared from MCF-7 cells at 2 µg
    Lane 3 : Whole cell lysate prepared from Rat1 cells at 2 µg
    Lane 4 : Whole cell lysate prepared from MDCK cells
    Lane 5 : Whole cell lysate prepared from MDCK cells
    Lane 6 : Whole cell lysate prepared from MDCK cells


    Predicted band size : 48 kDa
  • ab16144 at 1/50 dilution staining Calreticulin in human MCF-7 by Immunocytochemistry/ Immunofluorescence.

References for Anti-Calreticulin antibody (ab16144)

This product has been referenced in:
  • Gómez-Fernández C  et al. Relocalization of STIM1 in mouse oocytes at fertilization: early involvement of store-operated calcium entry. Reproduction 138:211-21 (2009). Read more (PubMed: 19470709) »

See 1 Publication for this product

Product Wall

ab22683's immunogen was Calreticulin-maltose binding fusion protein. I am very sorry but we have not done epitope mapping for this antibody and therefore cannot confirm exactly where it binds so unfortunately I cannot say whether it will recognise you...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"