Overview

  • Product name
    Anti-Calreticulin antibody
    See all Calreticulin primary antibodies
  • Description
    Goat polyclonal to Calreticulin
  • Specificity
    Recognises calreticulin.
  • Tested applications
    Suitable for: WB, IHC-Frmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Purified rabbit skeletal muscle calreticulin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    glycine buffered saline pH7.4, 0.1% azide
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/500.
IHC-Fr 1/50 - 1/70.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
    • Sequence similarities
      Belongs to the calreticulin family.
    • Domain
      Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
      The interaction with glycans occurs through a binding site in the globular lectin domain.
      The zinc binding sites are localized to the N-domain.
      Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
    • Cellular localization
      Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
    • Information by UniProt
    • Database links
    • Alternative names
      • Autoantigen RO antibody
      • CALR antibody
      • CALR protein antibody
      • CALR_HUMAN antibody
      • Calregulin antibody
      • Calreticulin antibody
      • cC1qR antibody
      • CRP55 antibody
      • CRT antibody
      • CRTC antibody
      • Endoplasmic reticulum resident protein 60 antibody
      • Epididymis secretory sperm binding protein Li 99n antibody
      • ERp60 antibody
      • FLJ26680 antibody
      • grp60 antibody
      • HACBP antibody
      • HEL S 99n antibody
      • RO antibody
      • Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody
      • Sicca syndrome antigen A antibody
      • SSA antibody
      see all

    References for Anti-Calreticulin antibody (ab4109)

    This product has been referenced in:
    • Lee H  et al. Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis. Nat Commun 7:12799 (2016). Read more (PubMed: 27619642) »

    See 1 Publication for this product

    Product Wall

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    20 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 01 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    20 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 01 2016

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Sample
    Rat Tissue lysate - whole (liver)
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 23 2014

    Thank you for contacting us.

    The size of antibody as IgG is 150 kDa (25 kDa of each light chain and ˜53 kDa of each heavy chain).

    The size of protein as mentioned before is 417 amino acid 48kDa.

    I hope this information is hel...

    Read More

    Thank you for contacting us.

    The predicted size of unprocessed protein is 48kDa (http://www.uniprot.org/uniprot/P27797). The observed size will be higher than predicted because of post translational modifications at amino acid 48,159, 209 and ...

    Read More

    Thank you for contacting us.

    The predicted size of unprocessed protein is 48kDa (http://www.uniprot.org/uniprot/P27797). The observed size will be higher than predicted because of post translational modification at amino acid 48,159, 209 and ...

    Read More

    Thank you for contacting us.

    The antibody ab2907 was tried in Arabidopsis by a customer, and the results were submitted as an Abreview (2 out of 5 stars): http://www.abcam.com/index.html?datasheet=2907&tab=abreviews&intabreviewid=249...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 03 2011

    This antibody has not been tested for use in ELISA assays. As it is supplied as a serum, it would not be suitable for use as a coating antibody. There is no immunological reason why it could not be used as a detection antibody, but we cannot guarantee ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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