Anti-Calreticulin antibody (ab70013)

Overview

  • Product nameAnti-Calreticulin antibody
    See all Calreticulin primary antibodies
  • Description
    Rabbit polyclonal to Calreticulin
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    synthesized peptide derived from an internal region of human Calreticulin

  • Positive control
    • Extracts from COS7 cells. Heart tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionMolecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • Sequence similaritiesBelongs to the calreticulin family.
  • DomainCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • Cellular localizationEndoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all

Anti-Calreticulin antibody images

  • All lanes : Anti-Calreticulin antibody (ab70013) at 1/500 dilution

    Lane 1 : extracts from COS7 cells
    Lane 2 : extracts from COS7 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 110 kDa,19 kDa,35 kDa. We are unsure as to the identity of these extra bands.
  • immunohistochemistry analysis of paraffin-embedded human heart tissue using 1/50 ab70013. Right: with pretreatment of the antibody with the immunizing peptide

References for Anti-Calreticulin antibody (ab70013)

ab70013 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. I have looked through our catalog for the products against the targets that you have chosen that either have been specifically tested in ELISA or are guaranteed to work in ELISA by us. My findings are as follows:

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Following experiments related to ELISA have been carried out: 1) immunogen peptide ELISA--coat immunogen peptide to 96-well plate and detect with ab70013 with/without peptide preincubation. 2) cell-based ELISA--grow cells in 96-well plate, fix the ce...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"