Overview

  • Product name
    Anti-Calreticulin antibody
    See all Calreticulin primary antibodies
  • Description
    Chicken polyclonal to Calreticulin
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Dog, Human
    Predicted to work with: Rabbit, Cow, Pig, Zebrafish, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse Calreticulin aa 24-43.
    Sequence:

    KEQFLDGDAWTNRWVESKHK


    (Peptide available as ab5838)

  • Positive control
    • AtT-20 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1mg/ml BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab37382-Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 60-66 kDa (predicted molecular weight: 48 kDa).Can be blocked with Calreticulin peptide (ab5838).
ICC Use at an assay dependent concentration.

Target

  • Function
    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • Sequence similarities
    Belongs to the calreticulin family.
  • Domain
    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • Cellular localization
    Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all

Images

  • All lanes : Anti-Calreticulin antibody (ab94935) at 1/2000 dilution

    Lane 1 : A-549
    Lane 2 : HEK 293
    Lane 3 : Mouse Brain

    Lysates/proteins at 30 µg per lane.

    Secondary
    Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate at 0.4 µg/ml

    Predicted band size : 48 kDa
  • Anti-Calreticulin antibody (ab94935) at 1/1000 dilution + AtT-20 cell lysate

    Predicted band size : 48 kDa

References

ab94935 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab94935.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up