Anti-Calreticulin antibody [EPR3925] - ER Marker (ab108395)


  • Product nameAnti-Calreticulin antibody [EPR3925] - ER Marker
    See all Calreticulin primary antibodies
  • Description
    Rabbit monoclonal [EPR3925] to Calreticulin - ER Marker
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Calreticulin.

  • Positive control
    • WB: Fetal brain, fetal kidney, and NIH 3T3 cell lysates IHC-P: Human thyroid gland tissue ICC/IF: HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR3925
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab108395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionMolecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
    • Sequence similaritiesBelongs to the calreticulin family.
    • DomainCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
      The interaction with glycans occurs through a binding site in the globular lectin domain.
      The zinc binding sites are localized to the N-domain.
      Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
    • Cellular localizationEndoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
    • Information by UniProt
    • Database links
    • Alternative names
      • Autoantigen RO antibody
      • CALR antibody
      • CALR protein antibody
      • CALR_HUMAN antibody
      • Calregulin antibody
      • Calreticulin antibody
      • cC1qR antibody
      • CRP55 antibody
      • CRT antibody
      • CRTC antibody
      • Endoplasmic reticulum resident protein 60 antibody
      • Epididymis secretory sperm binding protein Li 99n antibody
      • ERp60 antibody
      • FLJ26680 antibody
      • grp60 antibody
      • HACBP antibody
      • HEL S 99n antibody
      • RO antibody
      • Sicca syndrome antigen A antibody
      • SSA antibody
      see all

    Anti-Calreticulin antibody [EPR3925] - ER Marker images

    • All lanes : Anti-Calreticulin antibody [EPR3925] - ER Marker (ab108395) at 1/1000 dilution

      Lane 1 : Fetal brain
      Lane 2 : Fetal kidney
      Lane 3 : NIH 3T3 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 48 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of Calreticulin in paraffin embedded Human thyroid gland tissue, using ab108395 at a 1/100 dilution.
    • Immunofluorescent staining of Calreticulin in HeLa cells, using ab108395 at a 1/100 dilution.

    References for Anti-Calreticulin antibody [EPR3925] - ER Marker (ab108395)

    ab108395 has not yet been referenced specifically in any publications.

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