Overview

Description

  • NatureRecombinant
  • SourceNative

Specifications

Our Abpromise guarantee covers the use of ab15729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • FormLiquid
  • Additional Notes

    Purified full length rabbit Calreticulin protein. SwissProt ID P15253.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

General info

  • Alternative names
    • Autoantigen RO
    • CALR
    • CALR protein
    • CALR_HUMAN
    • Calregulin
    • Calreticulin
    • cC1qR
    • CRP55
    • CRT
    • CRTC
    • Endoplasmic reticulum resident protein 60
    • Epididymis secretory sperm binding protein Li 99n
    • ERp60
    • FLJ26680
    • grp60
    • HACBP
    • HEL S 99n
    • RO
    • Sicca syndrome antigen A
    • SSA
    see all
  • FunctionMolecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • Sequence similaritiesBelongs to the calreticulin family.
  • DomainCan be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • Cellular localizationEndoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Information by UniProt

Calreticulin full length protein images

  • Coomassie stain of SDS-PAGE gel with ab15729.

References for Calreticulin full length protein (ab15729)

This product has been referenced in:
  • Cheng WF  et al. Tumor-specific immunity and antiangiogenesis generated by a DNA vaccine encoding calreticulin linked to a tumor antigen. J Clin Invest 108:669-78 (2001). Read more (PubMed: 11544272) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry regarding this Calreticulin protein (ab15729). The MW of this recombinant protein is >47kDa, (however it migrates on SDS-PAGE as an approximately 60-kDa protein band). I would suggest loading 2-5 ug per lane. It will r...

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Dear Dr. Hill, Thank you for your patience. Below is the amino acid sequence for ab15729. This rabbit calreticulin protein carries a myc-tag and his-tag at the C-terminus. I hope this information helps with the Mass Spectroscopy experiments. ...

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Thank you for your enquiry and patience. Unfortunately, we don't have additional information regarding this product at this time. If we do receive such information, we will update the online datasheet. We do know that the recombinant calreticulin was e...

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Thank you for your email. The buffer only consists of PBS, and there are no preservatives in the buffer. If you have any more questions, please contact us again.

My apologies for the delay in replying to your enquiry, we have just received the following information from the source of ab15729: "The protein is in PBS and we have not added any Ca or Zn to this solution. One may want to add a small amount of EG...

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Thank you for your enquiry. Here are some details regarding this protein: Recombinant calreticulin was expressed in E. coli and purified as His-tag protein. Full length cDNA encoding mature rabbit calreticulin was cloned into pBAD expression vector fol...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"